Prospects and implications of using chromatin insulators in gene therapy and transgenesis

BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
F Recillas-TargaCatherine Farrell

Abstract

Gene therapy has emerged from the idea of inserting a wild-type copy of a gene in order to restore the proper expression and function of a damaged gene. Initial efforts have focused on finding the proper vector and delivery method to introduce a corrected gene to the affected tissue or cell type. Even though these first attempts are clearly promising, several problems remain unsolved. A major problem is the influence of chromatin structure on transgene expression. To overcome chromatin-dependent repressive transgenic states, researchers have begun to use chromatin regulatory elements to drive transgene expression. Insulators or chromatin boundaries are able to protect a transgene against chromatin position effects at their genomic integration sites, and they are able to maintain transgene expression for long periods of time. Therefore, these elements may be very useful tools in gene therapy applications for ensuring high-level and stable expression of transgenes.

References

Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M SadelainR C Mulligan
Mar 1, 1997·Nature Biotechnology·Y WangB W O'Malley
Oct 31, 1998·Current Opinion in Genetics & Development·C M Hart, U K Laemmli
Oct 29, 1998·Blood Cells, Molecules & Diseases·D W EmeryG Stamatoyannopoulos
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·F Recillas-TargaG Felsenfeld
Jan 5, 2000·Journal of Cellular Biochemistry·M Izumi, D M Gilbert
Jan 8, 2000·The Journal of Biological Chemistry·D A WilloughbyR G Oshima
Apr 8, 2000·Current Opinion in Genetics & Development·R Festenstein, D Kioussis
Jul 13, 2000·Biochemical and Biophysical Research Communications·W PottsC Martin
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D W EmeryG Stamatoyannopoulos
Sep 22, 2000·Plant Molecular Biology·G C AllenW F Thompson
Dec 7, 2000·Molecular Cell·T I GerasimovaV G Corces
Feb 13, 2001·Science·E MuravyovaP Georgiev
Aug 2, 2001·Reviews in Medical Virology·D Pannell, J Ellis
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·C FrancastelM Groudine
Feb 5, 2002·Genes & Development·Adam G WestGary Felsenfeld
Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David Donze, Rohinton T Kamakaka
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Félix Recillas-TargaGary Felsenfeld
Jun 14, 2002·Cell·Kojiro IshiiUlrich K Laemmli
Jun 18, 2002·Current Opinion in Cell Biology·Monika Lachner, Thomas Jenuwein
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bonnie Burgess-BeusseGary Felsenfeld

❮ Previous
Next ❯

Citations

Oct 5, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Sergey B AkopovEugene D Sverdlov
Nov 19, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Stefan Worgall
Sep 18, 2007·Molecular Biotechnology·Adam S Cockrell, Tal Kafri
Jun 14, 2005·Molecular and Cellular Endocrinology·Philip B DanielKathleen G Mountjoy
Apr 29, 2005·Cell Research·Gong Hong WeiChih Chuan Liang
Sep 16, 2009·Briefings in Functional Genomics & Proteomics·Eduardo MoltóLluís Montoliu
Jan 21, 2011·Nucleic Acids Research·Nickolai A TchurikovYuri V Kravatsky
May 25, 2006·Annual Review of Genomics and Human Genetics·Glenn A MastonMichael R Green
Nov 26, 2010·The Journal of Clinical Investigation·Patrick G GallagherDavid M Bodine
Jun 15, 2011·BMC Cancer·Mercedes Dávalos-SalasFélix Recillas-Targa
Dec 23, 2006·BMC Biotechnology·Nicole R LeitnerMarina Karaghiosoff
Jun 20, 2008·Genetics·Emily J Kuhn-ParnellPamela K Geyer
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Héctor Rincón-AranoFélix Recillas-Targa
Jan 21, 2010·Immunopharmacology and Immunotoxicology·Steven FroelichPin Wang
Aug 31, 2006·Expert Opinion on Emerging Drugs·Matthieu D Lavigne, Dariusz C Górecki
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yoshitaka MiyagawaJoseph C Glorioso
Jun 17, 2008·Lancet·Alain Fischer, Marina Cavazzana-Calvo
Mar 11, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Lluís MontoliuAngel García-Díaz
Feb 15, 2008·Experimental Cell Research·Urban DeutschBritta Engelhardt
Oct 10, 2006·Pediatric Clinics of North America·Joydeep Basu, Huntington F Willard
Dec 26, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Georgina GuerreroFélix Recillas-Targa
Jan 12, 2007·Genesis : the Journal of Genetics and Development·Hsu-Shui WuMeng-Chun Hu
Nov 14, 2006·Journal of Molecular Biology·Mathias Hampf, Manfred Gossen
Oct 12, 2005·Trends in Biotechnology·Cole FergusonCynthia E Dunbar
May 11, 2005·Trends in Molecular Medicine·Joydeep Basu, Huntington F Willard
Jul 3, 2013·Methods : a Companion to Methods in Enzymology·Hong Thi Tran, Kris Vleminckx
Apr 21, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arthur W NienhuisBrian P Sorrentino
Oct 16, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jean M LayLinda C Samuelson
Apr 6, 2005·Human Molecular Genetics·Adam G West, Peter Fraser
Mar 17, 2007·Cancer Research·Inti A De La Rosa-VelázquezFélix Recillas-Targa

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.