Prostacyclin analog-suppressed ischemia-reperfusion injury of the rat liver: evaluation by calpain mu activation

The Journal of Surgical Research
M WangM Monden

Abstract

Prostaglandin I2 has a protective effect on hepatic ischemia-reperfusion injury. However, the exact intracellular mechanisms of this effect have not been elucidated. Calpain micro, a Ca2+-dependent protease, has been found to play a role in the ischemia-reperfusion injury of various organs. The hilar area of the left lateral and median lobes of rat livers was clamped for 60 min. A prostaglandin I2 analog (OP2507, C35H41NO4) was intravenously administered at 0.1, 0.32, or 1.0 microg/kg/min from 20 min before the ischemia. In addition to biochemical and microscopic analyses, the activation of calpain mu was investigated using specific antibodies against the intermediate (activated) and preactivated forms of calpain mu. The degradation of talin was also studied by Western blotting. When OP2507 was infused at 0.32 and 1.0 microg/kg/min, bile flow significantly increased after reperfusion compared with the control group, consistent with the decrease in serum transaminase levels. Membrane bleb formation and the appearance of the intermediate form of calpain mu were observed at 60 min of ischemia in the control and OP2507 (0.1 microg/kg/min) groups and remained present until 120 min after reperfusion. OP2507 (1.0 microg/kg/min) marked...Continue Reading

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Citations

Jul 31, 2008·World Journal of Gastroenterology : WJG·Meng WangMeng-Chao Wu
Jan 11, 2005·World Journal of Gastroenterology : WJG·Qi-Ping Lu, Lei Tian
Aug 10, 2010·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Wilson J ChangLuis H Toledo-Pereyra
Jul 5, 2003·The Journal of Surgical Research·Kazunori TaniguchiMorito Monden

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