Prostacyclin analogs stimulate VEGF production from human lung fibroblasts in culture

American Journal of Physiology. Lung Cellular and Molecular Physiology
Koichiro KamioStephen I Rennard

Abstract

Prostacyclin is a short-lived metabolite of arachidonic acid that is produced by several cells in the lung and prominently by endothelial cells. It increases intracellular cAMP levels activating downstream signaling thus regulating vascular mesenchymal cell functions. The alveolar wall contains a rich capillary network as well as a population of mesenchymal cells, i.e., fibroblasts. The current study evaluated the hypothesis that prostacyclin may mediate signaling between endothelial and mesenchymal cells in the alveolar wall by assessing the ability of prostacyclin analogs to modulate fibroblast release of VEGF. To accomplish this study, human lung fibroblasts were cultured in routine culture on plastic support and in three-dimensional collagen gels with or without three prostacyclin analogs, carbaprostacyclin, iloprost, and beraprost, and the production of VEGF was evaluated by ELISA and quantitative real-time PCR. Iloprost and beraprost significantly stimulated VEGF mRNA levels and protein release in a concentration-dependent manner. These effects were blocked by the adenylate cyclase inhibitor SQ-22536 and by the protein kinase A (PKA) inhibitor KT-5720 and were reproduced by a direct PKA activator but not by an activator o...Continue Reading

References

Jun 15, 1989·Biochemical and Biophysical Research Communications·N Ferrara, W J Henzel
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Jul 1, 1996·In Vitro Cellular & Developmental Biology. Animal·T MioS I Rennard
Dec 6, 2000·The Journal of Clinical Investigation·Y KasaharaN F Voelkel
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·T KohyamaS I Rennard
May 30, 2002·American Journal of Respiratory Cell and Molecular Biology·Tadashi KohyamaStephen I Rennard
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tadashi KohyamaStephen I Rennard
Aug 3, 2002·American Journal of Respiratory and Critical Care Medicine·Sekiya KoyamaTakateru Izumi
Oct 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Richard StrattonAndrew Leask
Jun 18, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shingo YasuharaJ A Jeevendra Martyn
Jan 9, 2004·Experimental Lung Research·Takeo IshiiTakeshi Matsuse
Oct 2, 2004·The European Respiratory Journal·O HolzR A Jörres
Nov 24, 2004·Journal of the American College of Surgeons·Celine BuckleyRobert W Thompson
Feb 12, 2005·Respiratory Research·Fiorella CalabreseMarialuisa Valente
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bethany B MooreMarc Peters-Golden
May 17, 2005·Respiratory Medicine·Ellen Tufvesson, Leif Bjermer
Jul 23, 2005·Current Heart Failure Reports·Jalal K Ghali
Jan 13, 2006·Biochimica Et Biophysica Acta·H BachofenS Schürch
Jan 31, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carolyn J BagloleRichard P Phipps
Mar 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Koichiro KamioStephen I Rennard

❮ Previous
Next ❯

Citations

May 20, 2006·American Journal of Respiratory Cell and Molecular Biology·Carla M PradoIolanda F L C Tibério
Mar 6, 2010·American Journal of Respiratory Cell and Molecular Biology·Ying-Ji LiStephen I Rennard
Feb 3, 2012·American Journal of Respiratory Cell and Molecular Biology·Masanori NakanishiStephen I Rennard
May 10, 2013·American Journal of Respiratory Cell and Molecular Biology·Jun IkariStephen I Rennard
Oct 24, 2012·Pharmacology & Therapeutics·Bart G J DekkersMartina Schmidt
Feb 16, 2010·The Journal of Allergy and Clinical Immunology·Viktoria KonyaAkos Heinemann
Dec 3, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S PoulinM Rola-Pleszczynski
Oct 16, 2014·Pulmonary Pharmacology & Therapeutics·Louise M HarknessAnna-Karin Larsson-Callerfelt
Aug 27, 2014·Journal of Endodontics·Chalida Nakalekha LimjeerajarusPrasit Pavasant
Oct 23, 2013·Ultrastructural Pathology·Rosemary C Jones, Diane E Capen
Apr 20, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Kengo KanaiKazunori Nishizaki
Dec 3, 2014·Prostaglandins & Other Lipid Mediators·Ying-Ji LiStephen I Rennard
Aug 24, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Gunilla Westergren-ThorssonAnna-Karin Larsson-Callerfelt
May 19, 2011·Journal of Leukocyte Biology·Eva M SturmAkos Heinemann
May 27, 2017·Odontology·Thanomsuk JearanaiphaisarnChalida Nakalekha Limjeerajarus
Jun 29, 2013·International Journal of Oncology·Jia WangShin-Ichi Akiyama
Jul 1, 2017·American Journal of Respiratory and Critical Care Medicine·Shaney L BarrattAnn B Millar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.