Prostacyclin synthesis elicited by cholinergic agonists is linked to activation of M2 alpha and M2 beta muscarinic receptors in the rabbit aorta

Prostaglandins
N Jaiswal, K U Malik

Abstract

This study was conducted to investigate the subtypes of muscarinic receptors involved in the action of cholinergic agents on prostacyclin synthesis in the rabbit aorta. Prostacyclin production measured as 6-keto-PGF1 alpha was assessed after exposing the aortic rings to different cholinergic agents. Acetylcholine (ACh) (M1 and M2 agonist) (1-10 microM) and arecaidine proparagyl ester (APE) (M2 selective agonist) (1-10 microM) enhanced 6-keto-PGF1 alpha output in a concentration-dependent manner. A selective M1 receptor agonist, McN-A-343, at 1 microM-1 mM did not alter 6-keto-PGF1 alpha output. ACh- and APE induced increases in 6-keto-PGF1 alpha output were attenuated by the M1/M2 antagonist atropine (0.1 microM), M2 alpha antagonist (AF-DX 116), (0.1-1.0 microM), and by selective M2 beta antagonist, hexahydro-sila-difendiol (HHSiD) (0.1-1.0 microM), but not by the M1 antagonist pirenzepine (1.0 microM). 6-Keto-PGF1 alpha output elicited by ACh- or APE was not altered by the adrenergic receptor antagonists phentolamine and propranolol or by the nicotinic receptor blocker hexamethonium. Similarly, the arachidonic acid- or norepinephrine induced 6-keto-PGF1 alpha accumulation was not altered by these muscarinic receptor antagonis...Continue Reading

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Citations

Mar 17, 1999·British Journal of Pharmacology·A K HammarströmH A Coleman
May 15, 2007·European Journal of Pharmacology·Chiko ShinboriKeisuke Satoh
Jul 1, 1991·Hypertension·E A TallantC M Ferrario
Jul 19, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Xin TangWilliam B Campbell
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Francis A SylvesterJulian H Lombard
Mar 27, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Shane A PhillipsJulian H Lombard
Sep 27, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Shane A PhillipsJulian H Lombard
Mar 22, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ines Drenjancevic-PericJulian H Lombard

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