Prostaglandin A1 inhibits increases in intracellular calcium concentration, TXA(2) production and platelet activation

Acta Pharmacologica Sinica
Yi ZhuZheng-hong Qin

Abstract

In our previous studies we found that cyclopentenane prostaglandin A1 (PGA1) had neuroprotective effects in a rodent ischemic model. In the present study we aimed to investigate the inhibitory effect of PGA1 on platelet function. The rate of aggregation of human platelets was measured by using turbidimetry. The rate of adhesion of platelets to cultured endothelial cells was determined by using [(3)H]-adenine labeled platelets. 5-Hydroxytryptamine release from platelets was measured with O-phthaldialdehyde fluorospectrophotometry. The levels of TXB(2), a stable metabolite of TXA(2), were determined by radioimmuno-assay. Alternations in platelet morphology were observed using an electron microscope, and the intraplatelet free calcium concentrations were measured with Fluo-3/AM FCM assay. PGA1 significantly inhibited thrombin-, collagen- and ADP-induced aggregation and adhesion of platelets. The morphological changes of platelets induced by thrombin were blocked by PGA1. PGA1 inhibited the release of 5-hydroxytyptamine from dense granules and the synthesis of TXA(2). PGA1 inhibits the activation of platelets probably through blocking increases in intracellular calcium concentration and TXA(2) synthesis.

References

Mar 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A K Dutta-RoyA K Sinha
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M G SantoroC Amici
Sep 10, 1969·Biochemical and Biophysical Research Communications·N R MarquisP A Tavormina
Feb 1, 1982·The Tohoku Journal of Experimental Medicine·H HoshiaiM Suzuki
Oct 13, 1995·The Journal of Biological Chemistry·K YuM A Lazar
Sep 1, 1993·Baillière's Clinical Haematology·T F Lüscher
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·A RossiM G Santoro
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S C ThomasH R Wong
Apr 13, 2001·Medicinal Research Reviews·D S Straus, C K Glass
Nov 20, 2002·Journal of Neurochemistry·Xiaoxia WangM Catherine Bennett
Mar 8, 2003·Stroke; a Journal of Cerebral Circulation·Ludmila BelayevMyron D Ginsberg
Nov 1, 2003·Thrombosis Research·J van Gijn, A Algra
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Mar 23, 2005·Brain Research·Hui-Ling ZhangZheng-Hong Qin
Jun 1, 2005·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Temel TombulOmer Anlar
Jul 12, 2005·Cerebrovascular Diseases·Hon-Kan YipMien-Cheng Chen

❮ Previous
Next ❯

Citations

Jun 21, 2008·Acta Pharmacologica Sinica·Li ZhangZhong-qin Liang
Mar 30, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Fei GuoYang Wang
Jan 11, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Guo-Biao XuPu Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.