PMID: 2497784May 19, 1989Paper

Prostaglandin D2 receptor of mastocytoma P-815 cells--possible regulation by phosphorylation and dephosphorylation

Biochimica Et Biophysica Acta
S YoshimuraA Ichikawa

Abstract

The 3H-labeled prostaglandin D2 [( 3H]PGD2) binding protein in the membrane fraction of mastocytoma P-815 cells was characterized. The specific binding of [3H]PGD2 to the cells or the membranes reached a maximum at pH 5.6, and was saturable, displaceable and of high affinity when incubated at 0 or 37 degrees C. The Bmax values for [3H]PGD2 binding in the two preparations at pH 5.6 were much higher at 0 degrees C than at 37 degrees C, whereas the Kd values were almost equal (85.3 nM for the cells and 80.5 nM for the membranes, respectively). High specific [3H]PGD2 binding activity in the mildly acid-treated cells was still observed when the external pH was raised from 5.6 to 7.2. Furthermore, specific [3H]PGD2 binding to the membranes (at 0 degrees C, pH 5.6) increased on addition of phosphatase inhibitors (NaF and molybdate) in the presence of 10 microM ATP, but practically disappeared on pretreatment of the membranes with phosphatase. On incubation of the membrane with [gamma-32P]ATP and molybdate, the stimulated incorporation of the [32P]phosphate into several peptides, including ones having an Mr of around 100,000-120,000, was observed. These results suggest that [3H]PGD2 binding in the mastocytoma P-815 cell membrane is con...Continue Reading

References

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Citations

Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M HirataS Narumiya
Oct 1, 1991·Visual Neuroscience·S M Dawis
Aug 11, 1995·The Journal of Biological Chemistry·Y BoieM Abramovitz
Feb 1, 1994·Protein Science : a Publication of the Protein Society·K PalczewskiK A Walsh
Dec 7, 2005·British Journal of Pharmacology·Varsha IyerBrian J Knoll
May 1, 1995·Journal of Medical Genetics·M A MawA Gal
Oct 14, 2020·Investigative Ophthalmology & Visual Science·Juan C Valdez-LopezPhyllis R Robinson
Jan 1, 1993·Progress in Lipid Research·M NegishiA Ichikawa
Oct 26, 1991·Biochimica Et Biophysica Acta·H HashimotoA Ichikawa

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