Prostaglandin E production and viability of cells cultured in contact with freshly mixed endodontic materials

International Endodontic Journal
K K MelegariG R Holland

Abstract

To determine whether commonly used endodontic sealers could either induce or increase the release of prostaglandin E2 (PGE2) when in contact with cell types found in the periapical tissues. Freshly mixed samples of Roth 801 sealer, Sealapex and ProRoot mineral trioxide aggregate (MTA) were placed in contact with cultured macrophages and fibroblasts for 24 h. The supernatant from the cultures was assayed for PGE2 using enzyme-linked immunosorbent assay. Cell viability counts were made. As a positive control, similar cultures were also exposed to lipopolysaccharide and the supernatant analysed for PGE2. Data were compared by anova. The three materials examined in these experiments did not stimulate increased PGE2 release from either of the cell lines. In control cultures, lipopolysaccharide increased PGE2 release from macrophages but not from fibroblasts. Viability counts revealed that, whilst Roth 801 sealer caused some cell death in both fibroblasts and macrophages, Sealapex led to cell death only in the macrophage cultures. ProRoot MTA did not lead to statistically significant cell death in either culture. Under 24-h culture conditions, the three freshly mixed test materials did not increase directly either production or relea...Continue Reading

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Citations

Jun 25, 2009·International Endodontic Journal·F AbbasipourM Janahmadi
Dec 14, 2011·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Heloisa Fonseca MarãoPedro Ivo Santos Silva
Nov 15, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Wonkyung YangMiri Kim
Dec 23, 2009·Journal of Applied Oral Science : Revista FOB·Karin Cristina da Silva ModenaCarlos Ferreira Santos

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