PMID: 8589921Oct 1, 1995Paper

Prostaglandin E1 inhibits TNF alpha-induced T-cell adhesion to endothelial cells by selective down-modulation of ICAM-1 expression on endothelial cells

Experimental Dermatology
J M WeissJ C Simon

Abstract

Prostaglandins have been shown to be involved in the suppression of contact hypersensitivity (CHS) by so-far ill understood mechanisms. T-cell migration across the lining of cytokine-activated endothelial cells (EC) is thought to be a central step in the initiation of CHS. The aim of our investigation was therefore to examine whether prostaglandin E1 (PGE1) influences cytokine-induced TK-1 mouse T-cell lymphoma adhesion to eEnd.2 mouse endothelioma cells. Here, we report that PGE1 (10(-12)-10(-8) M) dose-dependently reduced TNF alpha-induced T-cell binding, while TNF alpha-unstimulated adhesion was not affected. To test whether PGE1 acted primarily on T-cells or on EC, they were separately pretreated with PGE1 prior to the adhesion assay. Selective PGE1 pretreatment of eEnd.2, but not of TK-1 dose-dependently inhibited TNF alpha, stimulated T-cell adhesion. Since binding of TK-1 to TNF alpha-treated eEnd.2 is mediated by the interaction of ICAM-1 and VCAM-1 (on EC) with their receptors LFA-1 and VLA-4 (on T-cells), we further investigated whether PGE1 would modulate the expression of these molecules. FACS-analysis revealed PGE1 to inhibit TNF alpha-induced upregulation of ICAM-1, but not of VCAM-1 on EC. Furthermore, constituti...Continue Reading

References

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Citations

Apr 8, 1998·Immunotechnology : an International Journal of Immunological Engineering·C C TermeerJ C Simon
Dec 9, 1998·Clinical and Experimental Immunology·C C TermeerJ C Simon
Aug 29, 2009·Journal of Atherosclerosis and Thrombosis·Shinji TakaiMizuo Miyazaki
Oct 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Hideo Kohka TakahashiMasahiro Nishibori
Aug 27, 2014·The Journal of Dermatology·Kazuhiko YamamuraSatoshi Takeuchi
Jul 28, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Youjia DuPeter Zahradka

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