Abstract
Prostaglandins have been shown to be involved in the suppression of contact hypersensitivity (CHS) by so-far ill understood mechanisms. T-cell migration across the lining of cytokine-activated endothelial cells (EC) is thought to be a central step in the initiation of CHS. The aim of our investigation was therefore to examine whether prostaglandin E1 (PGE1) influences cytokine-induced TK-1 mouse T-cell lymphoma adhesion to eEnd.2 mouse endothelioma cells. Here, we report that PGE1 (10(-12)-10(-8) M) dose-dependently reduced TNF alpha-induced T-cell binding, while TNF alpha-unstimulated adhesion was not affected. To test whether PGE1 acted primarily on T-cells or on EC, they were separately pretreated with PGE1 prior to the adhesion assay. Selective PGE1 pretreatment of eEnd.2, but not of TK-1 dose-dependently inhibited TNF alpha, stimulated T-cell adhesion. Since binding of TK-1 to TNF alpha-treated eEnd.2 is mediated by the interaction of ICAM-1 and VCAM-1 (on EC) with their receptors LFA-1 and VLA-4 (on T-cells), we further investigated whether PGE1 would modulate the expression of these molecules. FACS-analysis revealed PGE1 to inhibit TNF alpha-induced upregulation of ICAM-1, but not of VCAM-1 on EC. Furthermore, constituti...Continue Reading
References
Jun 1, 1992·Immunology Today·R PardiJ R Bender
Jan 21, 1992·International Journal of Cancer. Journal International Du Cancer·M BouillonM Audette
Mar 1, 1992·Immunology Today·Y ShimizuS Shaw
Mar 1, 1991·The Journal of Experimental Medicine·K MiyakeP W Kincade
Apr 26, 1990·The New England Journal of Medicine·M MoranO Jonasson
Mar 1, 1990·The Journal of Experimental Medicine·A H DingC F Nathan
Feb 1, 1987·Cellular Immunology·W V ScheuerW O Weigle
Aug 1, 1989·The Journal of Investigative Dermatology·D E Cavender
Apr 15, 1987·Cellular Immunology·L A RheinsJ J Nordlund
Mar 1, 1988·The Journal of Investigative Dermatology·B D JunR A Daynes
Apr 1, 1987·The Journal of Investigative Dermatology·B RobertsonR Daynes
Jan 15, 1988·Cell·R L WilliamsE F Wagner
Feb 25, 1974·Prostaglandins·F A Kuehl
May 1, 1993·The Journal of Cell Biology·M HahneD Vestweber
Jul 1, 1993·Cellular Immunology·J Marcinkiewicz, B M Chain
Citations
Jun 29, 1999·Surgery Today·H ItasakaK Sugimachi
Jan 13, 2001·Journal of Dermatological Science·M InaokiK Takehara
Apr 8, 1998·Immunotechnology : an International Journal of Immunological Engineering·C C TermeerJ C Simon
Dec 9, 1998·Clinical and Experimental Immunology·C C TermeerJ C Simon
Feb 12, 1998·Transplantation·S NatoriH Shimada
Aug 6, 2011·Lipids in Health and Disease·Christine DawczynskiGerhard Jahreis
Nov 11, 2010·Lipids in Health and Disease·Undurti N Das
Aug 29, 2009·Journal of Atherosclerosis and Thrombosis·Shinji TakaiMizuo Miyazaki
Nov 21, 2014·PloS One·Allan F WiechmannEric W Howard
Jun 29, 2014·Fish & Shellfish Immunology·Luke R IwanowiczVicki S Blazer
Oct 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Hideo Kohka TakahashiMasahiro Nishibori
Aug 27, 2014·The Journal of Dermatology·Kazuhiko YamamuraSatoshi Takeuchi
Jul 28, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Youjia DuPeter Zahradka
Dec 19, 2006·The Journal of Surgical Research·Tariq HafezAhmed Abdul-Hadi