Prostaglandin E2 Activates NLRP3 Inflammasome in Endothelial Cells to Promote Diabetic Retinopathy

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
Yixin WangYong Yao

Abstract

Proliferative diabetic retinopathy (PDR) is the leading cause of blindness and accounts for approximately 12% of all new cases of blindness. Prostaglandin E2 (PGE2) and nucleotide-binding domain and Leucine-rich repeat Receptor containing a Pyrin domain 3 (NLRP3) inflammasomes have been long considered to be associated with PDR. Levels of pro-inflammatory mediators were examined by Enzyme-linked immunosorbent assay (ELISA) measurements. PGE2 levels were analyzed by using the Prostaglandin E2 Monoclonal EIA Kit. Human retinal microvascular endothelial cells (HRMECs) were stained with Annexin V/propidium iodide and analyzed by flow cytometry for testing the apoptosis. Expression levels of NLRP3 inflammasome components under various conditions were detected by Western blot and real-time PCR. Inflammasome markers and PGE2 were highly expressed in the vitreous of PDR patients. PGE2 and NLRP3 induced apoptosis of HRMECs. PGE2 upregulated the expression of NLRP3 inflammasome components and inflammatory chemokines. Knockdown of NLRP3 attenuated the expression of NLRP3 inflammasome components and inhibited the effect of PGE2. Our results suggest that PGE2 levels and NLRP3 inflammasome activation are closely related to the pathogenesis of PDR a...Continue Reading

Citations

Jul 11, 2020·Medicinal Research Reviews·Xu WangGuang Liang
Apr 8, 2020·Oxidative Medicine and Cellular Longevity·Ricardo Raúl Robles-RiveraAdolfo Daniel Rodríguez-Carrizalez
Sep 20, 2020·Cell Death & Disease·Baochen BaiXian-Ming Chu
Oct 8, 2020·Ocular Immunology and Inflammation·Karanvir S Raman, Joanne A Matsubara
Aug 6, 2020·International Journal of Molecular Sciences·Jin-Seok LeeChang-Gue Son
Nov 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ana Carolina Guerta SalinaC Henrique Serezani
May 13, 2021·European Journal of Pharmacology·Abdullah Al MamunJian Xiao
Jan 11, 2022·Investigative Ophthalmology & Visual Science·Li LiuJena J Steinle

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