Prostaglandin E2 biphasic control of lymphocyte proliferation: inhibition by picomolar concentrations

FEBS Letters
M G SergeevaS D Varfolomeyev

Abstract

Prostaglandins (PGs) have an important physiological role in the modulation of various cell immune functions. The main sources of PGs during immune responses are monocyte cells. We report here the ability of non-stimulated macrophages to synthesize prostanoids and show that peritoneal mouse macrophages synthesize PGE2, PGF2a and thromboxane B2, spleen macrophages produce PGE2 and PGF2a, and in a fresh medium this synthesis reaches a constant basal level in a few hours. We studied the kinetics of Con A-induced proliferation of murine splenocytes under the influence of a wide range of PGE2 concentrations (10(-14)-10(-7) M). The suppressive effect of PGE2 decreased when its concentration was lowered and disappeared at 10(-9) M PGE2 (this concentration corresponded to the basal level of non-stimulated macrophage synthesis of PGE2). Further lowering of the concentration became essential for the proliferation process once again, and at picomolar concentrations PGE2 caused a suppressive effect comparable with that for 10(-8) M PGE2. We also found that PGE2 significantly inhibited cell proliferation when it was added 1 h before the addition of mitogen, as compared with simultaneous mitogen addition. The effect was obtained for both low...Continue Reading

References

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Citations

Sep 25, 2003·Toxicon : Official Journal of the International Society on Toxinology·Flavia GarciaLeonilda M B Santos
Oct 3, 1999·European Journal of Biochemistry·M GoncharS Varfolomeyev
Jul 15, 2003·Clinical Microbiology Reviews·Mairi C NoverrGary B Huffnagle
May 2, 2007·Infection and Immunity·John R Erb-Downward, Mairi C Noverr
Dec 19, 2001·Infection and Immunity·Mairi C NoverrGary B Huffnagle
Feb 22, 2003·Infection and Immunity·Mairi C NoverrGary B Huffnagle
Mar 25, 2004·FEMS Immunology and Medical Microbiology·Rut LucasM Luisa Gil
Mar 17, 2007·Mycopathologia·Ana Paula BordonAngela Maria Victoriano Campos de Soares
Mar 7, 2021·International Journal of Molecular Sciences·Anne-Kathrin KnuthAndreas Weigert
Mar 2, 1999·Molecular and Cellular Neurosciences·W D HirstG P Wilkin
May 16, 2002·International Immunopharmacology·Bianca Rocca, Garret A FitzGerald
Feb 13, 2001·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Y Suresh, U N Das
Jan 30, 2010·Toxicon : Official Journal of the International Society on Toxinology·Sandra C SampaioYara Cury

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