Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: an in vivo and in vitro study

Prostaglandins, Leukotrienes, and Essential Fatty Acids
G O Ramirez-YañezA L Symons

Abstract

The aims of this study were to determine how Prostaglandin E2 (PGE2) locally applied affected the immunodistribution of latent transforming growth factor-beta 1 (TGF-beta1), and how the eicosanoid modified TGF-beta1 release and TGF-beta receptors gene expression in cultured osteoblasts. PGE2 locally delivered on the rat mandible at doses of 0.1 and 0.05 mg/day, but not 0.025 mg/day, over 20 days significantly increased latent TGF-beta1 immunodistribution (P<0.001), comparing with a placebo-treated group. Cultured osteoblasts stimulated with 10(-5) or 10(-7)M PGE2 significantly varied the level of activated TGF-beta1 released into supernatants at different experimental periods compared with negative and positive controls. TGF-beta receptor type I gene expression was significantly increased in osteoblasts (P<0.01) after 10 days of treatment with 10(-5) and 10(-7)M PGE2, whereas 10(-3) M PGE2 produced the opposite effect. It is concluded that PGE2 may stimulate bone deposition by affecting TGF-beta pathway. This effect on the pathway appears to be dose-dependent.

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Dec 14, 2004·Bone·G O Ramirez-YañezA L Symons

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Citations

Jan 9, 2009·Annals of Biomedical Engineering·Lindsay A SharpAaron S Goldstein
Mar 12, 2010·Tissue Engineering. Part C, Methods·Emma L SmithAlastair J Sloan
Oct 3, 2014·Mediators of Inflammation·Qianqian Miao, Momar Ndao
Oct 9, 2008·Breast Cancer Research and Treatment·Antoinette R TanMichael Reiss
Jul 6, 2013·Nutrition Research·Owen J KellyJasminka Z Ilich
May 3, 2007·European Journal of Immunology·Carlos Ocaña-MorgnerAna Rodriguez
Mar 2, 2011·Journal of Applied Toxicology : JAT·Enrique ChávezPablo Muriel
Jun 8, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Enrique ChávezPablo Muriel
Apr 7, 2012·Archives of Oral Biology·G O Ramirez-Yanez, A L Symons
Dec 22, 2011·Experimental Biology and Medicine·Jung-Woo KangSun-Mee Lee
Feb 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhou WuHiroshi Nakanishi
Jun 30, 2021·Journal of Medical Microbiology·Jacobus T R BrinkCarolina H Pohl

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