Prostaglandin E2 induced caspase-dependent apoptosis possibly through activation of EP2 receptors in cultured hippocampal neurons

Neurochemistry International
Tsuneo TakaderaTakao Ohyashiki

Abstract

Cyclooxygenase-2 (COX-2) induction and prostaglandin E2 elevation have been reported to occur after cerebral ischemic insult. To evaluate whether the cyclooxygenase-2 reaction product prostaglandin E2 is directly related to induction of apoptosis in neuronal cells, the effect of prostaglandin E2 on cell viability was examined in hippocampal cells. Prostaglandin E2 (5-25 microM) induced apoptosis in a dose-dependent manner 48 h after addition to the cells, which was characterized by cell shrinkage, nuclear condensation or fragmentation and attenuated by a protein synthesis inhibitor, cycloheximide. Neither 17-phenyl trinor-prostaglandin E2 (an EP1 agonist) nor sulprostone (an EP3 agonist) induced cell death, whereas butaprost (an EP2 agonist) induced apoptosis. Prostaglandin E2 increased the intracellular concentration of cAMP, and the selective EP2 agonist butaprost also induced apoptosis accompanied by increasing cAMP levels in hippocampal cells. Moreover, a cell permeable cAMP analog, dibutyryl cAMP also induced apoptosis in hippocampal cells. These findings suggest that prostaglandin E2-induced apoptosis was mediated through a mechanism involving the cAMP-dependent pathway. In addition, prostaglandin E2 activated caspase-3 a...Continue Reading

References

Jan 1, 1993·Acta Neuropathologica·M O DereskiJ H Garcia
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M NakayamaS H Graham
Aug 11, 1999·Trends in Neurosciences·U DirnaglM A Moskowitz
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·C IadecolaM E Ross
Mar 30, 2001·The Journal of Biological Chemistry·S KimJ Chung
Jun 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Marina S FeschenkoKathleen J Sweadner
Jun 24, 2003·Nature Cell Biology·Marjorie MailletFrank Lezoualc'h
Nov 7, 2003·Neurochemistry International·Kazuhide KurosuYoshinori Kamisaki

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Citations

Jul 16, 2010·Journal of Neural Transmission·Michael T HenekaMarkus Peter Kummer
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·D V KryskoK D'Herde
Jun 16, 2010·Journal of Molecular Neuroscience : MN·Leila DargahiAbolhassan Ahmadiani
Jan 27, 2010·Neuroscience Bulletin·Li-Li WeiWei Chen
Dec 23, 2006·Journal of Neurotrauma·Abdullah Shafique AhmadSylvain Doré
Aug 30, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nan SangGuang-ke Li
Feb 19, 2009·Journal of Neuroinflammation·Noel G CarlsonJohn W Rose
Sep 3, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuri Ikeda-Matsuo
Jul 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuri Ikeda-MatsuoYasuharu Sasaki
Dec 23, 2015·Neurotoxicology·A K AshleyM E Legare
Jul 4, 2012·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Shahrzad Soleymani FardAli M Ardekani
Jul 7, 2012·Biochemical and Biophysical Research Communications·Yukiko KurokiShuntaro Hara
Dec 15, 2015·Biochimica Et Biophysica Acta·Ana B Fernández-MartínezFrancisco J Lucio Cazaña
Dec 1, 2009·Brain Research Reviews·Michael T HenekaAlexei Verkhratsky
Jun 16, 2009·Neurochemistry International·Belmira Lara da Silveira Andrade da CostaNeville N Osborne
Jan 14, 2009·Biochemical and Biophysical Research Communications·Min Kyu ParkKi Churl Chang
May 12, 2009·Neurochemistry International·Anthony J HutchinsonJohn W Regan
Jan 20, 2006·The European Journal of Neuroscience·Muzamil AhmadSylvain Doré
Aug 7, 2009·Journal of Neuroscience Research·Takako TakemiyaKanato Yamagata
Sep 6, 2005·Free Radical Biology & Medicine·Mei-Hua LiYoung-Joon Surh
Jun 21, 2005·European Journal of Pharmacology·Tania F GendronPaul Morley
Jan 31, 2013·Toxicology and Applied Pharmacology·Lauren M TetzRita Loch-Caruso
Oct 30, 2012·International Immunopharmacology·Silvia ReinaEnri Borda
Mar 22, 2013·Journal of Pharmacological Sciences·Hiroko MiyagishiYoshihisa Ito
Jan 10, 2008·International Immunopharmacology·Peter M BenzThomas Renné
Mar 8, 2011·Neurotoxicology·Dejan MilatovicMichael Aschner
Dec 3, 2014·Neurotoxicology·Shekher MohanSylvain Doré
May 28, 2016·Journal of Tissue Engineering and Regenerative Medicine·Anat YanaiKevin Gregory-Evans
Jan 1, 2010·Journal of Nutrigenetics and Nutrigenomics·Basant K Puri, Mehar S Manku
Sep 8, 2006·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Nan Sang, Chu Chen

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