Prostaglandin E2 induces up-regulation of murine macrophage beta-endorphin receptors

Immunology Letters
R A GelfandM R Jadus

Abstract

Cultured murine bone marrow macrophages specifically bound 125I-labeled beta-endorphin. Binding was displaceable by 100 times molar excess of full-length beta-endorphin but was insensitive to the opioid receptor antagonist, naloxone. Binding was inhibited by beta-endorphin's C-terminal tetrapeptide, lys-lys-gly-glu, but not by the truncated N-terminal 27 amino acid fragment, indicating that binding of beta-endorphin to this receptor is dependent on its C-terminus. Macrophages incubated for 24 h with 10(-8)-10(-5) M prostaglandin E2 showed a dose-dependent increase in beta-endorphin binding, implying receptor up-regulation. This was also observed in response to the phosphodiesterase inhibitor, isobutylmethylxanthine, indicating that regulation of these receptors may be mediated through a cAMP-dependent process. This is the first demonstration that beta-endorphin receptor expression can be positively regulated.

References

Sep 7, 1979·Science·E HazumP Cuatrecasas
Feb 1, 1991·Immunological Reviews·C J HeijnenR E Ballieux
Jan 1, 1990·Autoimmunity·J Watson, E S Wijelath
May 1, 1988·Journal of Neuroimmunology·W Gilmore, L P Weiner
Feb 1, 1985·Neuropeptides·M R RuffC B Pert
Jul 1, 1986·Journal of Neuroimmunology·D J Carr, G R Klimpel
Mar 1, 1987·Brain, Behavior, and Immunity·A W KusnecovR Smith
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L SchweigererC Gramsch
Nov 1, 1986·Cellular Immunology·S L Brown, D E Van Epps
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H M JohnsonJ E Blalock
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S C GilmanJ D Feldman
Jan 1, 1984·The Journal of Clinical Investigation·S J LolaitJ W Funder

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Citations

Mar 1, 1995·Endocrine·R A GelfandL Parker
Jun 4, 1998·Journal of Neuroimmunology·J J MaddenD Ketelsen
Jan 1, 1996·Peptides·G A OlsonA J Kastin
Mar 11, 1998·Peptides·S YehudaD I Mostofsky
Oct 24, 2002·Neurobiology of Aging·Shlomo YehudaDavid I Mostofsky
Mar 14, 2001·Drug and Alcohol Dependence·L McCarthyT J Rogers
Mar 10, 2001·Journal of Neuroimmunology·L McCarthyT J Rogers

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