Prostaglandin E2 produced by Entamoeba histolytica binds to EP4 receptors and stimulates interleukin-8 production in human colonic cells.

Infection and Immunity
Indranil Dey, Kris Chadee

Abstract

Entamoeba histolytica pathogenesis in the colon occurs in a stepwise fashion. It begins with colonization of the mucin layer, which is followed by stimulation of a proinflammatory response that causes nonspecific tissue damage that may facilitate parasite invasion of the underlying colonic mucosa. Unfortunately, the parasite and/or host factors that stimulate a proinflammatory response in the gut are poorly understood. In this study, we found that live E. histolytica or secretory or proteins (SP) and soluble ameba components (SAP) can markedly increase interleukin-8 (IL-8) mRNA expression and protein production in colonic epithelial cells. The IL-8-stimulating molecule produced by live amebae was identified as prostaglandin E(2) (PGE(2)) as trophozoites treated with cyclooxygenase inhibitors inhibited the biosynthesis of PGE(2) and eliminated IL-8 production induced by live parasites or ameba components. Moreover, using specific prostaglandin EP2 and EP4 receptor agonists and antagonists, we found that PGE(2) binds exclusively through EP4 receptors in colonic epithelial cells to stimulate IL-8 production. Silencing of EP4 receptors with EP4 small interfering RNA completely eliminated SP- and SAP-induced IL-8 production. These s...Continue Reading

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M LeippeH J Müller-Eberhard
Nov 1, 1987·The Journal of Clinical Investigation·W A PetriJ I Ravdin
Oct 25, 1996·Annals of the New York Academy of Sciences·C SvanborgB Wullt
Feb 7, 2001·Clinical Microbiology Reviews·M Espinosa-Cantellano, A Martínez-Palomo
Jun 8, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Y YuK Chadee
Mar 2, 2002·Immunology·Maureen N Ajuebor, Mark G Swain
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vadim IourgenkoMark A Labow
Oct 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Indranil DeyKris Chadee
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Martin E LidellGunnar C Hansson
Nov 29, 2007·Parasite Immunology·E A García-ZepedaP Ostoa-Saloma
Jan 9, 2008·Molecular and Biochemical Parasitology·María Esther Morales-MartínezGuadalupe Rico-Rosillo

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Citations

Jan 5, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Young Hee NamMyeong Heon Shin
Nov 2, 2012·Seminars in Immunopathology·Hans P VerkerkeChelsea S Marie
Jul 3, 2013·Microbes and Infection·Abdur RubYusuf Akhter
Sep 22, 2010·Infection and Immunity·Sandra LourenssenMichael G Blennerhassett
Feb 8, 2014·Parasite : Journal De La Société Française De Parasitologie·Young Ah LeeMyeong Heon Shin
Aug 16, 2011·Trends in Parasitology·Nobuko YoshidaMartin S Llewellyn
Jan 5, 2010·Developmental and Comparative Immunology·Timothée BruelFrançois Meurens
Aug 29, 2012·British Journal of Pharmacology·F Neuschäfer-RubeG P Püschel
Jul 16, 2015·Molecular Microbiology·Koji Watanabe, William A Petri
Mar 23, 2010·Experimental Parasitology·Leanne Mortimer, Kris Chadee
Dec 21, 2014·Prostaglandins & Other Lipid Mediators·Sonia Rehal, Pierre-Yves von der Weid
Jun 1, 2016·Frontiers in Immunology·Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
May 4, 2004·Biochemical and Biophysical Research Communications·Sungwoo RyooKwang-Lae Hoe
Jun 19, 2013·Pharmacological Reviews·Utako YokoyamaYoshihiro Ishikawa
Jun 4, 2020·Physiology·Eileen Uribe-Querol, Carlos Rosales
Jun 5, 2020·Journal of Leukocyte Biology·Sharmin BegumKris Chadee
Jan 29, 2019·Frontiers in Cellular and Infection Microbiology·Preeti ShahiKris Chadee
Aug 21, 2019·Infection and Immunity·Julieta Pulido-OrtegaJavier Ventura-Juárez
Mar 14, 2019·Cellular Microbiology·Mengyao Niu, Nancy P Keller
Oct 8, 2019·International Journal of Medical Microbiology : IJMM·Julio C CarreroMireya de la Garza
Jul 29, 2021·Journal of Surgical Case Reports·Jorge Alberto DorantesMaria G Zavala-Cerna

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