Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues--a comparative immunohistochemical study

Prostaglandins, Leukotrienes, and Essential Fatty Acids
S BiswasC A Paterson

Abstract

The purpose of the present study was to compare the localization of prostaglandin E(2) receptor subtypes in normal human and mouse ocular tissues. Paraffin embedded sections of normal human and mouse (129 Sv/Ev) eyes were treated with EP(1), EP(2), EP(3) and EP(4) specific antibodies and subsequently incubated with Alexa Fluor secondary antibody (Ex/Em=555/571) to detect the presence of EP receptor proteins. Fluorescence of the localized antibodies was visualized in a Carl Zeiss Microscope (Axiovert 200) and photographed using Carl Zeiss Axiocam camera. In mice EP(1) and EP(3) receptor subtypes were only moderately expressed, EP(3) receptor expression being almost negligible. In human cornea and iris ciliary body, EP(1) and EP(3) receptors were prominently expressed. EP(4) receptor was expressed moderately in human and mouse ocular tissues. EP(2) receptor was the most prominently and abundantly expressed receptor in both human and mouse ocular tissues. It is concluded that the pattern of the distribution of EP receptor subtypes in the ocular tissues are similar in human and mouse. Thus, 129 Sv/Ev strains of mice would make an appropriate animal model for studying the ocular pathophysiological roles of prostaglandin receptor ago...Continue Reading

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Citations

Feb 24, 2009·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·B'Ann True GabeltPaul L Kaufman
Sep 23, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Xiaohong LiXuyang Liu
Oct 11, 2012·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Sanae AbeTakeshi Yoshitomi
Apr 12, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Gina M YanochkoBart A Jessen
Jun 13, 2006·Ocular Immunology and Inflammation·S BiswasB H Koller
May 9, 2015·Seminars in Cancer Biology·Abbas K SamadiXujuan Yang
Sep 22, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Lorna M CryanColm O'Brien
Aug 8, 2009·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach
Jun 16, 2009·Neurochemistry International·Belmira Lara da Silveira Andrade da CostaNeville N Osborne
Aug 30, 2008·Progress in Retinal and Eye Research·Stephen Yazulla
Apr 3, 2007·Journal of Cataract and Refractive Surgery·Woojin LeeYoung Hoon Ohn
Aug 7, 2012·Acta Ophthalmologica·Sidse KringelholtToke Bek
Oct 21, 2006·Experimental Eye Research·S BiswasS Narumiya
Aug 15, 2006·Experimental Eye Research·A Rocha-SousaA F Leite-Moreira
Jan 29, 2013·Prostaglandins & Other Lipid Mediators·Iwona DamsAndrzej Kutner
Oct 21, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David F WoodwardCarol B Toris
Jul 11, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Masahiro FuwaJin-Zhong Zhang
Jul 7, 2021·Japanese Journal of Ophthalmology·Makoto Aihara
Oct 9, 2021·Journal of Food Biochemistry·Nazmun NaharNur Iliyani Mohd Ishak

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