Prostaglandin H synthase-2 gene regulation in the amnion at labour: histone acetylation and nuclear factor kappa B binding to the promoter in vivo

Molecular Human Reproduction
C M MitchellTamas Zakar

Abstract

Increased prostaglandin H synthase-2 (PGHS-2) expression in the amnion is critical for the production of prostaglandins that induce labour. The aim of the present investigation was to determine whether PGHS-2 gene activity is controlled by NFkappaB transcription factors in term amnion in vivo as suggested by in vitro findings. Amnion membranes were collected after elective Caesarean section (n = 14) or spontaneous labour (n = 12) at term, and histone acetylation and transcription factor binding to the PGHS-2 and IkappaBalpha promoters were determined in fresh tissues by chromatin immunoprecipitation. High level of histone-3 and -4 acetylation was detected in the proximal 1000 bp region of the PGHS-2 promoter indicating permissive chromatin structure in an area that contains two consensus NFkappaB binding sites and other transcription factor binding motifs. The TATA-box was occupied by TATA-binding protein (TBP) demonstrating that the PGHS-2 gene was transcriptionally active before and after labour. NFkappaB (p65 and p50) binding to the consensus sites, however, was detected only before, but not after, labour. Moreover, NFkappaB factor binding before labour was unrelated to TBP binding to the PGHS-2 TATA-box in the same tissues....Continue Reading

References

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Citations

Jan 27, 2010·Reproductive Biology and Endocrinology : RB&E·Sonali VoraWilliam E Ackerman
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Shigehiro MatsunagaHiroo Toyoda
Jan 22, 2017·Clinical and Experimental Pharmacology & Physiology·Kirsty G PringleEugenie R Lumbers
May 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deug-Chan LeeJung-Sun Kim
Dec 22, 2020·Frontiers in Physiology·Tamas Zakar, Jonathan W Paul
Apr 28, 2021·Molecular Reproduction and Development·Sheryl K MunroAnna P Ponnampalam

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