Prostaglandin-stimulated bone resorption by rheumatoid synovia. A possible mechanism for bone destruction in rheumatoid arthritis.

The Journal of Clinical Investigation
D R RobinsonL Levine

Abstract

Synovial tissue from patients with rheumatoid arthritis was maintained in organ culture for 3-14 days. Conditioned media from these synovial cultures contained bone resorption-stimulating activity, measured in vitro by using calcium release from mouse calvaria as the assay system. The synovial cultures also produce prostaglandin E2 (PGE2) as measured by serologic methods. The production of both the bone resorption-stimulating activity and PGE2 was inhibited by more than 90% by treatment of the synovial cultures with indomethacin (5 mug/ml). In contrast, treatment of the synovial cultures with colchicine (0.1 mug/ml) caused a marked and parallel increase in the concentration of both bone resorption-stimulating activity and PGE2 in the conditioned media. The bone resorption-stimulating activity was quantitatively extracted into diethyl ether. Within the limits of experimental error, all of the bone resorption-stimulating activity in medium was accounted for by its content of PGE2, itself a potent osteolytic factor. We conclude that the bone resorption-stimulating activity produced by rheumatoid synovia in culture is PGE2.

References

Jun 13, 1975·Annals of the New York Academy of Sciences·D R RobinsonL Levine
Apr 1, 1969·Arthritis and Rheumatism·E D HarrisS M Krane
Jun 1, 1970·Endocrinology·D C Klein, L G Raisz
Jan 20, 1972·The New England Journal of Medicine·G Weissmann
Aug 1, 1974·The Journal of Clinical Investigation·V A Ferraris, F R DeRubertis
Apr 18, 1974·The New England Journal of Medicine·G R MundyD N Buell

❮ Previous
Next ❯

Citations

Nov 11, 1999·Journal of Biomedical Materials Research·L C JonesD S Hungerford
Apr 1, 1977·Arthritis and Rheumatism·G HusbyR C Williams
Sep 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C B LangmanF Glorieux
May 15, 2001·Biochemical and Biophysical Research Communications·Y TsubouchiH Sano
Jan 1, 1984·Rheumatology International·H IshikawaK Hirohata
Jan 1, 1981·Springer Seminars in Immunopathology·J D Smiley, W L Hoffman
Jul 1, 1982·Agents and Actions·I G OtternessJ G Lombardino
Oct 1, 1986·Agents and Actions·M L Barrett, G P Lewis
Feb 28, 1978·Calcified Tissue Research·A CaniggiaS Bombardieri
Dec 8, 1978·Calcified Tissue Research·F K NorthingtonR E Wuthier
Jan 1, 1983·Archives of Gynecology·K JohH P Zahradnik
Mar 1, 1988·Clinical Rheumatology·J W Bijlsma
Jan 1, 1983·Calcified Tissue International·R M DziakE Hausmann
Jan 1, 1980·Calcified Tissue International·J H Dominguez, G R Mundy
Jan 1, 1981·Calcified Tissue International·A J Kahn, S L Teitelbaum
Jul 1, 1982·Calcified Tissue International·B E KreamA L Sandberg
Apr 1, 1991·Calcified Tissue International·M C Jungkeit, R A Chole
Feb 23, 1981·Biochimica Et Biophysica Acta·C J MalemudA Hassid
Dec 14, 1978·Biochemical and Biophysical Research Communications·A H Tashjian, L Levine
Jan 1, 1994·The International Journal of Biochemistry·A E Page, M J Warburton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.