Prostaglandins influence protein phosphorylation in established insect cell line

Archives of Insect Biochemistry and Physiology
David StanleyQisheng Song

Abstract

Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulating protein functions in eukaryotic cells and for some mammalian proteins it is influenced by PGs. We posed the hypothesis that PGs influence protein phosphorylation within insect cells, which we tested with the established insect cell line, BCIRL-HzAM1. After 20, 30, or 40 min incubations in the presence of one of three PGs (at 15 μM), PGA2 , PGE1 , or PGF2α , separate sets of cells were processed for analysis by two-dimensional electrophoresis followed by tandem mass spectrometry. We recorded significant phosphorylation changes in 31 proteins, decreases in 15, and increases in 15, and one protein with increased or decreased phosphorylation, depending on PG treatment. Increasing PG exposure times led to changes in fewer proteins, 20 min incubations led to changes in 16 proteins, 30 min to changes in 13, and 40 min to changes in 2 proteins. The proteins were identified by bioinformatic analyses, including transcrip...Continue Reading

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Citations

Oct 11, 2020·Archives of Insect Biochemistry and Physiology·Shabbir AhmedYonggyun Kim
Mar 20, 2021·Developmental and Comparative Immunology·Md Abdullah Al BakiYonggyun Kim
Aug 14, 2021·Developmental and Comparative Immunology·Shabbir Ahmed, Yonggyun Kim
Aug 28, 2021·Insects·Cynthia L GoodmanDavid Stanley
Sep 15, 2021·Archives of Insect Biochemistry and Physiology·Yong WangDavid Stanley

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