Prostanoid receptors EP2, EP4, and FP are regulated by estradiol in bovine oviductal smooth muscle

Prostaglandins & Other Lipid Mediators
Na HuangJinshan Cao

Abstract

Gamete and embryo transport is an important function of the oviduct. This transport involves both smooth muscle contraction and epithelial cell secretions, the former of which is mediated by prostaglandins (PGs) and their receptors. Our aim was to study the regulation of prostaglandin E2 and prostaglandin F2α receptors (EP2, EP4, and FP receptor) by estradiol in bovine oviduct smooth muscle. EP2, EP4, and FP receptor mRNA and protein expression was investigated using real-time RT-PCR and Western blot analyses, respectively. To evaluate the contraction or relaxation of cultured bovine oviductal smooth muscle tissue, peristalsis was used to assess contractile activity. EP2, EP4, and FP receptor mRNA and protein expression was increased in oviductal smooth muscle tissue after treatment with different concentrations of estradiol for various durations. The expression of all receptors peaked at an estradiol concentration of 10(-11)mol/L after 8h of treatment, whereas no increase in expression was observed after fulvestrant (a selective antagonist of E2 receptor) treatment, indicating that E2 interacts with specific E2 nuclear receptors to regulate EP2, EP4, and FP receptor expression. Although PGF2α and PGE2 induced both contraction ...Continue Reading

References

Jan 1, 1979·Gynecologic and Obstetric Investigation·S CaschettoL Wilhelmsson
Jan 1, 1975·Gynecologic Investigation·C H Spilman, M J Harper
Jan 1, 1975·Gynecologic Investigation·M Ahlgren
Mar 1, 1986·Prostaglandins, Leukotrienes, and Medicine·R B HowardM H Maguire
Feb 1, 1985·International Journal of Andrology·E BendvoldS Norén
Oct 1, 1972·The Journal of Endocrinology·B D MasonW D Kitts
Jan 1, 1983·General Pharmacology·I Stoyanov, A Nikolov
Feb 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Orlicky, C Williams-Skipp
Apr 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T MorishitaH Nakano
Apr 1, 1997·Fertility and Sterility·K OzgürO Erman
Jul 8, 1998·Prostaglandins & Other Lipid Mediators·J ChenJ Senior
Nov 5, 1999·Journal of Pediatric and Adolescent Gynecology·L Mastroianni
Sep 11, 2001·The Journal of Clinical Endocrinology and Metabolism·S A MilneH N Jabbour
Sep 24, 2004·Trends in Endocrinology and Metabolism : TEM·H N Jabbour, K J Sales
Aug 31, 2006·Prostaglandins & Other Lipid Mediators·S Pérez MartínezA Franchi
Nov 7, 2006·Prostaglandins & Other Lipid Mediators·Jinshan CaoTetsuro Taneike
Jul 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Suranga P KodithuwakkuMissaka P B Wijayagunawardane
Jan 20, 2012·Reproductive Biology and Endocrinology : RB&E·Chellakkan S BlessonLena Sahlin

❮ Previous
Next ❯

Citations

Nov 3, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hye-Ryun KimHaengseok Song
Nov 23, 2016·The Journal of Endocrinology·Shuai Li, Wipawee Winuthayanon
Aug 2, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Paula MoyanoJavier Del Pino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.