Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation

American Journal of Physiology. Renal Physiology
Li-Mei ChenKarl X Chai

Abstract

Prostasin is a glycosylphosphatidylinositol-anchored serine protease, with epithelial sodium channel activation and tumor invasion suppression activities. We identified the bladder as an expression site of prostasin. In the mouse, prostasin mRNA expression was detected by reverse transcription and real-time polymerase chain reaction in the bladder, and the prostasin protein was localized by immunohistochemistry in the urothelial cells. In mice injected intraperitoneally with bacterial lipopolysaccharide (LPS), bladder prostasin mRNA expression was downregulated, whereas the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interferon-gamma (IFN-gamma), TNF-alpha, IL-1beta, and IL-6 was upregulated. Viral promoter-driven expression of the human prostasin homolog in the bladder of transgenic mice attenuated the LPS induction of iNOS but did not abolish the induction. LPS induction of COX-2, TNF-alpha, IL-1beta, and IL-6 expression, however, was not reduced by prostasin transgene expression. Liposome-mediated delivery of prostasin-expressing plasmid into mouse bladder produced similar attenuation effects on LPS-induced iNOS expression, while not affecting COX-2 or cytokine induction. Mice receiving pl...Continue Reading

References

Aug 1, 1993·The Journal of Experimental Medicine·Y VodovotzC Nathan
Jan 1, 1997·The Journal of Clinical Investigation·M A WheelerW C Sessa
Apr 20, 2000·Journal of the American Society of Nephrology : JASN·Grégoire VuagniauxEdith Hummler
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G H CaugheyG M Verghese
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·M A MulveyS J Hultgren
Mar 29, 2001·The Journal of Biological Chemistry·L M ChenK X Chai
May 22, 2001·Cellular and Molecular Life Sciences : CMLS·B Walker, J F Lynas
Jun 27, 2001·Trends in Molecular Medicine·M B ChancellorJ Huard
Jan 5, 2002·The Journal of Biological Chemistry·Scott H DonaldsonSherif E Gabriel
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Li-Mei Chen, Karl X Chai
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Véronique ValletBernard C Rossier
Dec 13, 2002·American Journal of Physiology. Cell Physiology·Kimberly Palmiter LittlefieldSanford I Bernstein
Aug 5, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaonan HanRussell L Delude
Aug 30, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaonan HanRussell L Delude
Sep 10, 2003·American Journal of Respiratory Cell and Molecular Biology·George M VergheseGeorge H Caughey
Sep 13, 2003·European Journal of Pharmacology·Rebecka JohanssonKatarina Persson
Mar 3, 2004·The Prostate·Li-Mei ChenKarl X Chai
Apr 15, 2004·The Journal of Pharmacology and Experimental Therapeutics·Walter S KangRobert M Weiss
May 18, 2004·Trends in Immunology·Giuseppina Bonizzi, Michael Karin
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huiqing FangSusheela Tridandapani
Sep 9, 2004·Current Opinion in Obstetrics & Gynecology·Mat H HoOmid Khorram
Mar 25, 2005·Journal of Internal Medicine·O Wiedow, U Meyer-Hoffert
Aug 3, 2005·The Journal of Cell Biology·Céline LeyvrazEdith Hummler

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Citations

Mar 6, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Aya KodaKenichiro Kitamura
Oct 31, 2008·American Journal of Physiology. Renal Physiology·Renee E YuraJudith S Bond
Sep 21, 2013·Pflügers Archiv : European journal of physiology·Kristina Kusche-VihrogHans Oberleithner
Jun 28, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hai-Yan LinCheng Zhu
Mar 13, 2014·Nature Communications·Kohei UchimuraKenichiro Kitamura
Jun 11, 2010·Clinical and Experimental Nephrology·Kenichiro Kitamura, Kimio Tomita
Sep 1, 2012·Metabolism: Clinical and Experimental·William G WithamKenneth R McGaffin
May 22, 2010·The Journal of Urology·Clay W MechlinBadar M Mian
Jan 1, 2013·Journal of Biomarkers·Shakti AggarwalSarika Arora
Dec 19, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Hala El-MesallamyNada Osama
Apr 28, 2016·Clinical and Experimental Pharmacology & Physiology·D GiglioL Ny
Jul 16, 2014·Experimental Eye Research·Panagiotis LaspasAdrian Gericke
Dec 8, 2010·Neurosurgery·Leena PaulJogi V Pattisapu
May 3, 2018·American Journal of Physiology. Renal Physiology·Mariana G de OliveiraEdson Antunes
Nov 27, 2018·The Journal of Infectious Diseases·Chun-Kuang LinJin-Ching Lee
Sep 29, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Svetlana NikolaevaRimma Parnova
Feb 14, 2013·Experimental and Therapeutic Medicine·Abidin YildirimTurgut Dönmez
Nov 17, 2009·Molecular and Cellular Biochemistry·Mengqian ChenKarl X Chai
Oct 21, 2020·Journal of Ovarian Research·Mina SharbatoghliZahra Madjd
Jul 10, 2021·Bioscience Reports·Li-Mei ChenKarl X Chai

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