Prostasome-like particles are involved in the transfer of P25b from the bovine epididymal fluid to the sperm surface

Molecular Reproduction and Development
Gilles Frenette, Robert Sullivan

Abstract

In bulls, P25b is a sperm protein associated with the plasma membrane covering the acrosome. The amount of P25b bound to a constant number of spermatozoa varies from one individual to the other, low levels being associated with bull subfertility. In this study, we describe the epididymal origin of P25b using Western blot analysis. Whereas P25b was undetectable on caput spermatozoa, the amount of P25b associated to a constant number of spermatozoa increases from the corpus to the cauda. Prostasome-like particles were prepared by ultracentrifugation of epididymal fluid. P25b appears to be also associated with those membranous vesicles in increasing amounts along the epididymis. P25b is anchored to the plasma membrane of spermatozoa through glycosylphosphatidyl-inositol as shown by the ability of phospholipase C. but not of high salt treatment, to release P25b. Coincubation experiments revealed that prostasome-like particles are able to transfer P25b to spermatozoa, this process being more efficient at slightly acidic pH. P25b thus appears to be a marker of sperm epididymal maturation in bulls.

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Citations

Sep 5, 2002·Molecular Reproduction and Development·Hanae RejrajiJoël R Drevet
Dec 7, 2007·Histochemistry and Cell Biology·Beate WilhelmGerhard Aumüller
Oct 27, 2004·Cell and Tissue Research·Apichart NgernsoungnernPrapee Sretarugsa
May 30, 2013·Cell and Tissue Research·A SchwarzB Wilhelm
Oct 27, 2009·Molecules and Cells·Jeong Su OhChunghee Cho
May 17, 2005·Blood Cells, Molecules & Diseases·Robert SullivanGilles Frenette
Mar 3, 2006·Biology of Reproduction·Hanae RejrajiJoël R Drevet
Feb 1, 2008·Reproductive Biology and Endocrinology : RB&E·Laura SicilianoAmalia Carpino
Oct 25, 2012·Journal of Lipid Research·Rok KeberSimon Horvat
Nov 21, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Elizabeth G Bromfield, Brett Nixon
May 5, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Virginie Barraud-LangeAhmed Ziyyat
Apr 25, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Robert Sullivan, Fabrice Saez
Oct 24, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Marian AalbertsWillem Stoorvogel
Jun 26, 2007·Asian Journal of Andrology·Robert SullivanJulie Girouard
Nov 3, 2010·Asian Journal of Andrology·Fabrice SaezJoël R Drevet
Oct 29, 2010·Veterinary Medicine International·Julieta CaballeroRobert Sullivan
Dec 15, 2015·Human Reproduction Update·Ronit MachtingerAndrea A Baccarelli
Jun 9, 2004·Reproduction in Domestic Animals = Zuchthygiene·R El-Hajj GhaouiW M C Maxwell
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Jan 1, 2005·Theriogenology·Jean-Louis DacheuxFrançoise Dacheux
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Jul 24, 2004·Animal Reproduction Science·R Sullivan
Sep 7, 2004·Molecular Reproduction and Development·Rapeepun VanichviriyakitPrasert Sobhon
Oct 1, 2004·Molecular Reproduction and Development·Gilles FrenetteRobert Sullivan
Dec 8, 2007·Journal of Cellular Physiology·Kathleen M BaskaPeter Sutovsky
Apr 4, 2008·Molecular Reproduction and Development·Genevieve S GriffithsPatricia A Martin-Deleon

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