Prostate cancer-associated autoantibodies in serum against tumor-associated antigens as potential new biomarkers

Journal of Proteomics
Ramesh UmmanniStefan Balabanov

Abstract

The limitations of the current prostate cancer (PCa) screening tests demands new biomarkers for early diagnosis of PCa. In this study, we aim to investigate serum autoantibody signatures as PCa specific biomarkers. PCa proteins were resolved by 2-DE and then transferred onto polyvinylidene difluoride membrane, which were subsequently incubated with either pooled serum from PCa patients or from normal controls. Mass spectrometry results have identified 18 antigens from 21 different 2-DE spots associated with PCa. Autoantibody response to antigens PRDX2, PRDX6 and ANXA11 in PCa patient's sera was confirmed using recombinant antigens. Further validation with an independent set of PCa patient's sera have shown relatively increased abundance of PRDX6 and ANXA11 antibodies in PCa patients. Formal concept analysis method was applied to assess whether the abundance of these autoantibodies could influence the classification of patients. However, sensitivity of the single antibody to discriminate prostate tumor and healthy controls varies from 70% to 80%, whereas combination of both PRDX6 and ANXA11 antibodies increased sensitivity to 90% for tumors and 100% for healthy controls. Therefore, we hereby report that the detection of these an...Continue Reading

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Citations

Oct 5, 2016·Journal of Proteome Research·Elisa PinJochen M Schwenk
Apr 17, 2019·Journal of Food and Drug Analysis·Yi-Ting ChenYing-Hsu Chang
Oct 28, 2020·Technology in Cancer Research & Treatment·Eda G Ramirez-VallesCarlos A Alba-Fierro
Dec 19, 2020·Journal for Immunotherapy of Cancer·Hemanth K PotluriDouglas G McNeel
Jan 12, 2020·Seminars in Immunology·Makoto KobayashiSamir M Hanash
Aug 27, 2021·The Journal of Clinical Investigation·Rebecca A PorrittMoshe Arditi

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