Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles

Biochemical and Biophysical Research Communications
Dan StrattonJameel M Inal

Abstract

MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMs(-1) over 11 s, reaching a peak [Ca(2+)]i of 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMs(-1) over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at ∼ 430 nM.

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Apr 18, 2013·Biochimica Et Biophysica Acta·Jameel M InalSigrun Lange
Oct 11, 2014·Biochemical and Biophysical Research Communications·Dan StrattonJameel Inal

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Citations

Sep 19, 2018·Biomolecules·Hélène PolletDonatienne Tyteca
Aug 29, 2018·Frontiers in Pharmacology·Uchini S KosgodageSigrun Lange
Feb 1, 2020·Journal of Extracellular Vesicles·Mariadelva Catalano, Lorraine O'Driscoll
Sep 23, 2020·Pharmacology & Therapeutics·Maria Azparren-AnguloJuan M Falcon-Perez
Apr 13, 2021·Frontiers in Molecular Biosciences·Raeesah HayatudinNurolaini Kifli

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