Abstract
Previous studies revealed that prostate-specific antigen (PSA) is present in > 30% of human breast tumor cytosols. Survival analysis showed that patients with PSA-producing tumors have a reduced risk for relapse, suggesting PSA to be an independent favorable prognostic marker for a large subset of breast cancer patients. The present investigation established an in vivo model for the induction of PSA in human breast cancer tumors growing as xenografts in severe combined immunodeficient (SCID) mice. The human mammary cancer cell-line T47D was grown i.m. in female mice. When the tumor and leg diameter reached 10 mm, the mice were stimulated daily with norgestrel for either 5 or 7 days to produce PSA, and sacrificed on day 8. The prostate cancer cell-line LNCaP was grown in male mice and functioned as a positive control for PSA production. After T47D and LNCaP mice were sacrificed, a highly sensitive immunofluorometric assay was used to analyze the PSA concentration in the tumor, muscle, liver, and kidney cytosols. Norgestrel-stimulated T47D mice showed significantly more PSA in the tumors compared to tumors of the control mice. However, PSA levels in tumors of the stimulated mice were significantly lower than those in the LNCaP xe...Continue Reading
References
Jan 28, 1992·Biochemistry·C Y YoungD J Tindall
Oct 1, 1992·The Journal of Clinical Endocrinology and Metabolism·P CohenR G Rosenfeld
Jun 25, 1992·The New England Journal of Medicine·W L McGuire, G M Clark
May 1, 1991·The Journal of Urology·J E Oesterling
Apr 10, 1989·FEBS Letters·P H RiegmanJ Trapman
Feb 10, 1989·Cell·M Beato
Apr 1, 1994·Clinical Biochemistry·H YuD J Sutherland
Jan 1, 1994·Breast Cancer Research and Treatment·H YuL Grass
Jan 1, 1994·Breast Cancer Research and Treatment·E P DiamandisD J Sutherland
Jan 1, 1995·Journal of Clinical Laboratory Analysis·M LevesqueE P Diamandis
May 6, 1995·Lancet·E P Diamandis, H Yu
May 1, 1995·The Journal of Clinical Endocrinology and Metabolism·E P Diamandis, H Yu
Jan 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·J Malm, H Lilja
Jul 1, 1993·The Journal of Clinical Endocrinology and Metabolism·H KanetyA Karasik
Aug 4, 1993·Journal of the National Cancer Institute·G GaspariniA L Harris
Jun 11, 1996·International Journal of Cancer. Journal International Du Cancer·L C LaiR T Peaston
Jan 1, 1997·British Journal of Cancer·N ZarghamiE P Diamandis
Citations
Apr 7, 2000·Breast Cancer Research and Treatment·M H Black, E P Diamandis
Jan 6, 2001·Breast Cancer Research and Treatment
Nov 27, 2001·Critical Reviews in Clinical Laboratory Sciences·C BeckerH Lilja
Jan 10, 2008·International Journal of Cancer. Journal International Du Cancer·Joanna M DayAtul Purohit