Prostatic intraepithelial neoplasia and well differentiated adenocarcinoma maintain an intact basement membrane

Pathology, Research and Practice
David G BostwickA A Sinha

Abstract

The status of the basement membrane in prostatic intraepithelial neoplasia (PIN) and adenocarcinoma is unsettled. Previous studies using antibodies directed against Type IV collagen have been hindered by intense staining around the stromal smooth muscle fibers, making interpretation of acinar staining difficult. We employed a monoclonal antibody to heparan sulfate proteoglycan (HSPG) to overcome this problem, recognizing that HSPG is present in the basement membrane of epithelial and endothelial cells, but not stromal smooth muscle cells. In 22 totally-embedded whole mount radical prostatectomies for adenocarcinoma which contained PIN, intense HSPG immunoreactivity was observed in the basement membrane of all normal and hyperplastic acini, 98% of acini with high grade PIN, and 100% of acini of well differentiated (Gleason score 5) adenocarcinoma; vessels served as the internal positive control, with consistent staining throughout each specimen. The extent of HSPG immunoreactivity in cancer decreased with increasing Gleason grade (measured as percent of acini staining, in 10% increments; p = 0.002). These findings indicate that HSPG is a consistent component of the basement membrane of benign, hyperplastic, and early neoplastic ...Continue Reading

References

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Citations

Sep 19, 1997·The Prostate·D G BostwickA Lopez-Beltran
Apr 15, 2014·The Urologic Clinics of North America·David M Berman, Jonathan I Epstein
Sep 25, 2012·The Journal of Urology·Hugh J Lavery, Michael J Droller
Jul 26, 2008·Journal of Cellular Biochemistry·Norman J Maitland, Anne T Collins
Jun 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Norman J Maitland, Anne T Collins
Dec 24, 2002·Prostate Cancer and Prostatic Diseases·J S A GreenA V Kaisary

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