Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Pathways

Plant Molecular Biology Reporter
Mariangela CoppolaRosa Rao

Abstract

Systemin is a signal peptide that promotes the response to wounding and herbivore attack in tomato. This 18-amino acid peptide is released from a larger precursor, prosystemin. To study the role of systemin as a modulator of defense signaling, we generated tomato (Solanum lycopersicum) transgenic plants that overexpress the prosystemin cDNA. We carried out a transcriptomic analysis comparing two different transgenic events with the untransformed control. The Gene Ontology categories of the 503 differentially expressed genes indicated that several biological functions were affected. Systemin promotes the expression of an array of defense genes that are dependent on different signaling pathways and it downregulates genes connected with carbon fixation and carbohydrate metabolism. These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected. We show also that the observed transcriptional modifications have a relevant functional outcome, since transgenic lines were more resistant against very different biotic stressors such as aphids (Macrosiphum...Continue Reading

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Citations

Nov 16, 2017·Scientific Reports·Mariangela CoppolaGiandomenico Corrado
May 13, 2018·International Journal of Molecular Sciences·M Estrella SantamariaIsabel Diaz
Sep 16, 2018·International Journal of Molecular Sciences·Mariangela CoppolaEmilio Guerrieri
Nov 24, 2017·Protein Science : a Publication of the Protein Society·Martina BuonannoSimona Maria Monti
Jun 17, 2020·Frontiers in Plant Science·Julia Pastor-FernándezVíctor Flors
Dec 9, 2017·Chemical Society Reviews·Fernanda Oliveira ChagasMônica Tallarico Pupo
Jun 19, 2019·The New Phytologist·Yusuke Saijo, Eliza Po-Iian Loo

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Methods Mentioned

BETA
transgenic
PCRs
Feature Extraction
PCR
glycosylation
phosphotransferase

Software Mentioned

LOWESS
BlastX
InterProScan
ANNEX
Feature Extraction
Genespring
Blast2GO

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