Protamine assembled in multilayers on colloidal particles can be exchanged and released

Biomacromolecules
Sabine HillerEdwin Donath

Abstract

Biocomposite thin films assembled on colloidal particles by means of layer-by-layer adsorption have been suggested as drug carriers and diagnostic devices. Protamine (PRM)/dextransulfate (DXS) and protamine/bovine serum albumine (BSA) multilayers were fabricated on colloidal silica and subsequently investigated by means of fluorescence activated cell sorting (FACS) and microelectrophoresis. Fluorescein labeled polyelectrolytes were embedded at different positions in the multilayers as a marker for layer growth. FACS showed that PRM and DXS formed regular growing stable multilayers, yet adsorbed PRM can be nevertheless exchanged with PRM in solution during layer formation and also after the multilayer formation has been completed. Up to 90% of the PRM pool was available for exchange. PRM together with BSA as demonstrated by SFM did not form multilayers under the applied conditions although the zeta-potential, commonly used as an indicator for stepwise adsorption, observed characteristic alternations. The capability of bound PRM to exchange with PRM in solution is attributed to its relatively small size. The demonstrated exchange may have importance in designing multilayers with smart release features. Furthermore, FACS proved to...Continue Reading

References

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Citations

Oct 15, 2013·Colloids and Surfaces. B, Biointerfaces·Sha Wang, Zhiguang Guo
Mar 8, 2011·Biomacromolecules·Marie-Luce De TemmermanStefaan C De Smedt
Dec 12, 2012·Biomacromolecules·Jennifer FlemkeVolker Sieber
Oct 22, 2010·Journal of Biomaterials Science. Polymer Edition·Uta ReibetanzBjörn Neu
Jan 28, 2012·Chemical Society Reviews·Stefaan De KokerBruno G De Geest
Dec 16, 2005·Angewandte Chemie·Martin FischlechnerEdwin Donath
Feb 4, 2006·Macromolecular Bioscience·Uta ReibetanzEdwin Donath
Jan 23, 2007·Soft Matter·Andreas SchnäckelEdwin Donath
Aug 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Vincent StrehlowUta Reibetanz
Sep 25, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Ana Cláudia SantosFrancisco J Veiga
Apr 22, 2010·Biomacromolecules·Zhen SheGleb B Sukhorukov
Dec 15, 2010·Biomacromolecules·Jacqueline LessigUta Reibetanz
May 20, 2015·ACS Applied Materials & Interfaces·Ana C SantosAntónio J Ribeiro

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