Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine

Molecular Pharmaceutics
G PurasJ L Pedraz

Abstract

The present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors to analyze the potential application of this novel ternary formulation to deliver the pCMS-EGFP plasmid into the rat retina. Binary vectors based on niosome/DNA and ternary vectors based on protamine/DNA/niosomes were prepared and physicochemically characterized. In vitro experiments were performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, the resulted ternary vectors had 150 nm size, positive charge, spherical morphology, and condensed, released, and protected the DNA against enzymatic digestion. The presence of protamine in the ternary vectors improved transfection efficiency, cell viability, and DNA condensation. After ocular administration, the EGFP expression was detected in different cell layers of the retina depending on the administration route without any sign of toxicity associated with the formulations. While subretinal administration transfected mainly photoreceptors and retinal pigment epithelial cells at the site of injection, intravitreal administration produced a more uniform distribution of the protein expression through the inner layers of the retina. The protein expression in the retina persisted for...Continue Reading

References

Jan 1, 1969·Physics in Medicine and Biology
May 29, 1999·Bioconjugate Chemistry·P Midoux, M Monsigny
Mar 8, 2000·Nature·P Smaglik
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·I M Verma
Nov 15, 2002·Nature·Erika Check
Mar 5, 2003·Trends in Molecular Medicine·Gabriele D Schmidt-Wolf, Ingo G H Schmidt-Wolf
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
Jun 16, 2004·International Journal of Pharmaceutics·Shigeru KawakamiHitoshi Sasaki
Jun 23, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maytal Bivas-BenitaGerrit Borchard
Mar 25, 2005·Gene Therapy·S KachiP A Campochiaro
Nov 11, 2006·Advanced Drug Delivery Reviews·C BloquelF Behar-Cohen
Jan 20, 2007·Biochemical Society Transactions·L PeetersS C De Smedt
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Lap Thi NguyenHiroshi Kiwada
May 1, 2007·International Journal of Pharmaceutics·A del Pozo-RodríguezJ L Pedraz
Oct 5, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Baichao MaHongtao Lv
Jul 10, 2008·The Journal of Gene Medicine·Maayan Duvshani-EshetMarcelle Machluf
Aug 12, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Suna Choi, Kyung-Dall Lee
Oct 16, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·A del Pozo-RodríguezJ L Pedraz
May 11, 2010·Progress in Retinal and Eye Research·Shannon M Conley, Muna I Naash
Aug 25, 2010·The British Journal of Ophthalmology·Melissa M LiuChi-Chao Chan
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Jul 6, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Diego DelgadoAlicia Rodríguez-Gascón
Jul 13, 2011·Clinical & Experimental Ophthalmology·Peter Charbel Issa, Robert E MacLaren
Feb 11, 2012·Science Translational Medicine·Jean BennettAlbert M Maguire
May 10, 2012·Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery·Nelson Adami AndreolloLuiz Roberto Lopes
Jul 4, 2012·Current Opinion in Microbiology·Jason Mercer, Ari Helenius
Sep 22, 2012·Progress in Retinal and Eye Research·Daniel M LipinskiRobert E MacLaren
Dec 4, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·G PurasJ L Pedraz
Jan 12, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Michelle E McClements, Robert E MacLaren
Nov 16, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·G PurasJ L Pedraz
Dec 18, 2013·The Journal of Gene Medicine·Aritz Perez Ruiz de GaribayRenate Fuchs
Dec 30, 2014·International Journal of Pharmaceutics·Josune TorrecillaAlicia Rodríguez-Gascón
Jan 17, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jeffrey Adijanto, Muna I Naash

❮ Previous
Next ❯

Citations

Jul 19, 2016·Expert Opinion on Drug Delivery·Larissa Gomes Dos ReisDaniela Traini
Oct 12, 2018·The AAPS Journal·Brittany E GivensAliasger K Salem
Jul 17, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jiahui WangZhimin He
Jul 25, 2021·International Journal of Molecular Sciences·Myriam Sainz-RamosJose Luis Pedraz
Mar 30, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohamed MashalJose Luis Pedraz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
G PurasJ L Pedraz
European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
G PurasJ L Pedraz
European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
G PurasJ L Pedraz
© 2021 Meta ULC. All rights reserved