Proteae: a reservoir of class 2 integrons?

The Journal of Antimicrobial Chemotherapy
Ana Mendes MoreiraOlivier Barraud

Abstract

Our aim was to confirm with a large panel of clinical isolates that class 2 integrons are highly prevalent in Proteae and to analyse their genetic characteristics. Proteae (Proteus spp., Morganella spp. and Providencia spp.) isolates were collected from clinical samples during 2013 at Limoges University Hospital, France. The presence of class 1, 2 and 3 integrons was investigated by quantitative PCR. The presence of a stop codon in the intI2 gene was determined by Sanger sequencing. The gene cassette arrays of class 2 integrons were determined by PCR-RFLP and Sanger sequencing or next-generation sequencing when needed. Of the 327 Proteae collected, 103 (31.5%) harboured a class 2 integron and 45 (13.8%) a class 1 integron. No class 3 integrons were detected. One functional IntI2 integrase was detected in a Morganella morganii isolate. Six different gene cassette arrays were detected. Four had already been described in the literature: dfrA1-sat2-aadA1 (72 isolates), dfrA1-catB2-sat2-aadA1 (17), sat2-aadA1 (6) and lnu(F), dfrA1, aadA1 (1). We identified two new gene cassette arrays: (i) a new variant of the dfrA1 gene cassette (one isolate; the one with the functional IntI2); and (ii) the array dfrA1-gcu115-sat2 harbouring the ne...Continue Reading

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Citations

Jan 29, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jorge Erick Otero-OlarraAbigail Pérez-Valdespino
Feb 6, 2021·Current Microbiology·Xiaotong WangQuhao Wei
Oct 21, 2020·The Science of the Total Environment·Abigail Pérez-ValdespinoItza Mendoza-Sanchez
Jan 21, 2021·Antimicrobial Agents and Chemotherapy·Rong Xiang, Meng Li
Sep 25, 2021·The Journal of Antimicrobial Chemotherapy·Olivier BarraudUNKNOWN Iris Study Group

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