Protease activity of Blastocystis hominis

Parasitology Research
Selena W S SioKevin S W Tan

Abstract

Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.

References

Jan 1, 1986·Molecular and Biochemical Parasitology·H Scholze, E Werries
May 1, 1987·Molecular and Biochemical Parasitology·B C LockwoodG H Coombs
Jul 1, 1995·International Journal for Parasitology·A G Williams, G H Coombs
Jan 1, 1994·Methods in Enzymology·H Scholze, E Tannich
Jan 1, 1994·Methods in Enzymology·M J North
Aug 1, 1994·Archives of Pediatrics & Adolescent Medicine·Y S al-TawilK E Bommer
Jan 1, 1993·Annual Review of Microbiology·J H McKerrowJ Bouvier
Oct 1, 1996·Clinical Microbiology Reviews·D J Stenzel, P F Boreham
Jan 5, 2002·Acta Tropica·Hande DagciSeza Budak
Feb 19, 2002·Molecular and Biochemical Parasitology·M Sajid, J H McKerrow
May 19, 2004·Trends in Parasitology·Hisao YoshikawaTetsuo Hashimoto
Nov 30, 2004·Veterinary Parasitology·Kevin S W Tan
Sep 10, 2005·Parasitology Research·Manoj K PuthiaKevin S W Tan

❮ Previous
Next ❯

Citations

Oct 11, 2008·Parasitology Research·Haris Mirza, Kevin S W Tan
Feb 1, 2011·Parasitology Research·Dina Marie Abdel-Hameed, Omayma Mohamed Hassanin
Feb 7, 2014·Parasites & Vectors·Arutchelvan Rajamanikam, Suresh Kumar Govind
Feb 19, 2014·Parasitology Research·R FayerM Santin
Mar 10, 2012·Parasitology International·Ivan WawrzyniakHicham El Alaoui
Jul 30, 2014·The Journal of Eukaryotic Microbiology·Ishan K VyasFrancine Marciano-Cabral
May 25, 2016·Infection and Immunity·John Anthony YasonKevin Shyong Wei Tan
Feb 23, 2019·PloS One·Arutchelvan RajamanikamSuresh Kumar
Jul 26, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H MirjalaliM R Zali

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.