PMID: 9543717Apr 17, 1998Paper

Protease activity of Clostridium difficile strains

Canadian Journal of Microbiology
I PoilaneAnne Collignon

Abstract

The production of proteolytic enzymes by 10 Clostridium difficile isolates of varying toxigenicity and clinical origin was studied to determine if all isolates secreted proteases. Different protease substrates were studied: gelatin, collagen, phenylazobenzyloxycarbonyl-leucyl-glycyl-L-prolyl-D-arginine (Pz-peptide), casein, azocasein, and azocoll. All isolates degraded gelatin, collagen, and azocoll. The supernatants of all isolates contained an enzyme capable of attacking gelatin incorporated in a polyacrylamide gel (zymograms) and forming two closely spaced lytic bands with an estimated molecular mass of 35-40 kDa. Polyclonal antibodies, produced against the C. difficile gelatinase, revealed in Western blots a 35-kDa protein in the culture supernatants of all C. difficile isolates. In the same manner, Clostridium perfringens collagenase polyclonal antibodies detected a 120-kDa protein in the culture supernatants of all isolates; this suggests that at least two proteases may exist in C. difficile. The protease activities of the 10 strains examined did not seem strikingly different quantitatively but were in general weak and their role in pathogenicity is suspect.

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Citations

Sep 9, 2011·Folia Microbiologica·Vincenzo PasqualeKarel Krovacek
Nov 11, 2010·The Journal of Biological Chemistry·Yi-Pin LinYung-Fu Chang
Oct 17, 2006·Journal of Bacteriology·Sarah JacksonWilliam T Self
Jun 29, 2002·Journal of Clinical Microbiology·Tuomo KarjalainenAnne Collignon
Apr 24, 2014·Critical Reviews in Microbiology·Ana Sofia DuarteAna Cristina Esteves
Jul 18, 2009·International Journal of Antimicrobial Agents·C DenèveA Collignon
Jan 24, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Sawsan TahaKarel Krovacek
Mar 23, 2013·Cellular Microbiology·Lorenza TulliRosanna Leuzzi
Sep 3, 2013·Journal of the Science of Food and Agriculture·Mony R ChuonToshiki Enomoto
Oct 7, 2004·Pathologie-biologie·C JanoirA Collignon
Dec 17, 2014·Journal of Microbiological Methods·Changhoon ChaiSe-Wook Oh
Aug 24, 2012·Future Microbiology·Mahfuzur R Sarker, Daniel Paredes-Sabja
Jul 28, 2016·Nature Reviews. Microbiology·Carey D NadellKevin R Foster
Feb 13, 2001·Microbiology·C HennequinT Karjalainen
May 16, 2008·Journal of Medical Microbiology·Cécile DenèveClaire Janoir

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