PMID: 11342045May 9, 2001Paper

Protease C2, a cysteine endopeptidase involved in the continuing mobilization of soybean beta-conglycinin seed proteins

Biochimica Et Biophysica Acta
S SeoK A Wilson

Abstract

The protease that degrades the beta subunit of the soybean (Glycine max (L.) Merrill) storage protein beta-conglycinin was purified from the cotyledons of seedlings grown for 12 days. The enzyme was named protease C2 because it is the second enzyme to cleave the beta-conglycinin storage protein, the first (protease C1) being one that degrades only the alpha' and alpha subunits of the storage protein to products similar in size and sequence to the remaining intact beta subunit. Protease C2 activity is not evident in vivo until 4 days after imbibition of the seed. The 31 kDa enzyme is a cysteine protease with a pH optimum with beta-conglycinin as substrate of 5.5. The action of protease C2 on native beta-conglycinin produces a set of large fragments (52-46 kDa in size) and small fragments (29-25 kDa). This is consistent with cleavage of all beta-conglycinin subunits at the region linking their N- and C-domains. Protease C2 also cleaves phaseolin, the Phaseolus vulgaris vicilin homologous to beta-conglycinin, to fragments in the 25-28 kDa range. N-Terminal sequences of isolated beta-conglycinin and phaseolin products show that protease C2 cleaves at a bond within a very mobile surface loop connecting the two compact structural dom...Continue Reading

References

Nov 1, 1992·The Plant Journal : for Cell and Molecular Biology·A GranellJ Carbonell
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M P ScottN C Nielsen
Jul 1, 1990·Plant Molecular Biology·F L SebastianiR N Beachy
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Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
May 1, 1996·Phytochemistry·A L Tan-WilsonK A Wilson

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Citations

Jul 31, 2004·Plant Physiology and Biochemistry : PPB·Neel G BarnabyAnna Tan-Wilson
May 15, 2002·Biochimica Et Biophysica Acta·Patrick M BoydKarl A Wilson
Oct 8, 2013·Bioscience, Biotechnology, and Biochemistry·Andrei D ShutovNobuyuki Maruyama
Jun 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mireia TomasSílvia Atrian
Oct 20, 2011·Physiologia Plantarum·Liana TsiatsianiFrank Van Breusegem
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Amandeep SinghImtaiyaz Hassan
Sep 1, 2015·Plant Physiology and Biochemistry : PPB·Karl A Wilson, Anna Tan-Wilson
Oct 25, 2011·Physiologia Plantarum·Anna L Tan-Wilson, Karl A Wilson

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