PMID: 9420247Jan 7, 1998Paper

Protease cleavage of reovirus capsid protein mu1/mu1C is blocked by alkyl sulfate detergents, yielding a new type of infectious subvirion particle

Journal of Virology
K Chandran, M L Nibert

Abstract

Mammalian reovirus virions undergo partial disassembly of the outer capsid upon exposure to proteases in vitro, producing infectious subvirion particles (ISVPs) that lack protein sigma3 and contain protein mu1/mu1C as endoprotease-generated fragments mu1delta/delta and phi. ISVPs are thought to be required for two early steps in reovirus infection: membrane penetration and activation of the particle-bound viral transcriptase complexes. Genetic and biochemical evidence implicates outer-capsid protein mu1 in both these steps. To determine whether the cleavage of mu1/mu1C is relevant to the unique properties of ISVPs, we analyzed the properties of novel subvirion particles that lacked sigma3 yet retained mu1/mu1C in an uncleaved but cleavable form. These detergent-plus-protease subvirion particles (dpSVPs) were produced by treating virions with chymotrypsin in the presence of micelle-forming concentrations of alkyl sulfate detergents. Infections with dpSVPs in murine L or canine MDCK cells provided evidence that the cleavage of mu1/mu1C during viral entry into these cells is dispensable for reovirus infection. Additionally, dpSVPs behaved like ISVPs in their capacity to permeabilize lipid bilayers and to undergo transcriptase acti...Continue Reading

References

Dec 1, 1975·Virology·H D KlenkJ Blödorn
Apr 1, 1990·Molecular Microbiology·W A CramerH Y Song
Apr 1, 1991·Journal of Virology·M L NibertB N Fields
Mar 28, 1991·European Journal of Biochemistry·J H LakeyF Pattus
Nov 1, 1989·Journal of Virology·D K BodkinB N Fields
Jan 5, 1989·Nature·M W ParkerD Tsernoglou
Jan 1, 1985·Annual Review of Genetics·W K Joklik
Oct 1, 1986·Journal of Virology·D R ClevelandS Millward
Apr 22, 1986·Biochemistry·R BlumenthalI Pastan
Oct 1, 1972·Journal of Virology·A J Shatkin, A J LaFiandra
Mar 1, 1972·Virology·S C SilversteinG Acs
Dec 1, 1971·Virology·C T Chang, H J Zweerink
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S C SilversteinG Acs
Sep 1, 1981·Journal of Virology·S M ClarkR S Spendlove
Jan 1, 1982·Journal of Virology·D Drayna, B N Fields
Nov 1, 1982·The Journal of General Virology·D Drayna, B N Fields
Nov 1, 1982·The Journal of General Virology·D Drayna, B N Fields
Jan 15, 1981·Virology·P W LeeW K Joklik
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M T TostesonB N Fields
Mar 1, 1995·Current Biology : CB·F M Hughson
Sep 1, 1993·Journal of Virology·P Lucia-JandrisB N Fields
Feb 1, 1993·Analytical Biochemistry·B Vulliez-Le Normand, J L Eiselé
Oct 6, 1997·Journal of Virology·C L LuongoM L Nibert

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Citations

Dec 13, 2003·The Journal of Biological Chemistry·Joseph W GoldenLeslie A Schiff
Mar 7, 2015·The Journal of General Virology·Shicui YanQin Fang
May 3, 2002·The Journal of Biological Chemistry·Daniel H EbertTerence S Dermody
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Melina A AgostoMax L Nibert
Jul 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Melina A AgostoMax L Nibert
Jan 20, 2015·Annual Review of Virology·Charles C BaileyMichael Farzan
Jan 6, 2006·Rapid Communications in Mass Spectrometry : RCM·Ita HadzisejdićKevin M Coombs
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Sonja KubickiKarl-Erich Jaeger
Jan 5, 2018·Journal of Virology·Anthony J Snyder, Pranav Danthi
Sep 9, 2017·Scientific Reports·Megan L StaniferSteeve Boulant
Jul 5, 2002·Journal of Virology·Joseph W GoldenLeslie A Schiff

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