Protease-defective, gp120-containing human immunodeficiency virus type 1 particles induce apoptosis more efficiently than does wild-type virus or recombinant gp120 protein in healthy donor-derived peripheral blood T cells.

Journal of Clinical Microbiology
M KameokaK Ikuta

Abstract

Apoptosis and syncytium formation are two mechanisms by which human immunodeficiency virus type 1 (HIV-1) impairs uninfected CD4+ T-cell function and are mainly involved in the progression of the disease to AIDS. Previously, we showed that gp120-containing, protease-deficient HIV-1 (L-2) particles generated syncytia by particle-mediated fusion with uninfected cultured CD4+ T cells. Here, we present evidence that such L-2 particles can induce apoptosis in 40 to 50% of T cells which were enriched from HIV-1-negative healthy donor-derived peripheral blood mononuclear cells (PBMC-Ts). Activation of PBMC-Ts with phytohemagglutinin, concanavalin A, or ionomycin after incubation with L-2 particles resulted in the loss of proliferative capacity and gradual induction of apoptosis over 3 days. Wild-type strain LAI particles or recombinant gp120 were markedly less efficient (< or = 15%) at inducing such apoptosis. Western blot (immunoblot) analysis revealed that L-2 particles contained a larger amount of Env gp120 than LAI particles. Either preincubation of PBMC-Ts with a Fas antagonist or preincubation of L-2 particles with soluble CD4 blocked most of the apoptosis. This suggests that L-2-like particles can play a major role in HIV-1-ind...Continue Reading

References

Oct 1, 1992·The Journal of Experimental Medicine·N K BandaT H Finkel
Dec 1, 1991·Virology·A G Laurent-CrawfordA G Hovanessian
Jan 1, 1995·The Journal of Experimental Medicine·M R AldersonD H Lynch
Dec 1, 1995·Immunology Today·D H LynchM R Alderson

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Citations

Dec 11, 2013·Retrovirology·Jérôme EstaquierMireille Laforge
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haitao HuDrew Weissman
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·J J McSharry
Jul 16, 1999·Biochemical and Biophysical Research Communications·M KameokaK Yoshihara

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