Protease detection using a porous silicon based Bloch surface wave optical biosensor

Optics Express
Hong QiaoPeter J Reece

Abstract

In this article we present an optical biosensor for label-free detection of trace levels of protease activity. The scheme is based on surface functionalized porous silicon optical structures which supports optical Bloch surface modes. The optical structure provides a resonant optical mode for high sensitivity detection and open access of the sensing layer to the target enzyme. Protease detection is based on the digestion of gelatin, covalently attached inside the pore space, resulting in a spectral blue-shift of the optical mode. Monitoring of spatially separated resonant optical modes is used to eliminate optical response from nonspecific adsorption.

References

Nov 5, 1997·Science·V S LinM R Ghadiri
Jul 26, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Michael P SchwartzMichael J Sailor
Jan 24, 2007·Analytical Chemistry·Huimin OuyangPhilippe M Fauchet
Nov 1, 2007·ACS Nano·Kristopher A KilianJ Justin Gooding
Jul 1, 2010·Optics Express·Fabrizio GiorgisFrancesco Michelotti

❮ Previous
Next ❯

Citations

Aug 28, 2014·ACS Applied Materials & Interfaces·Giorgi ShtenbergEster Segal
Nov 12, 2013·Biosensors & Bioelectronics·Gilberto A RodriguezSharon M Weiss
Feb 29, 2020·Optics Letters·Michal GrygaPetr Hlubina
Jun 12, 2021·ACS Sensors·Mohamed A EttabibJames S Wilkinson
Nov 10, 2018·Analytical Chemistry·Sofia Arshavsky-GrahamSharon Weiss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.

Related Papers

Physical Chemistry Chemical Physics : PCCP
Francesco MichelottiFrancesco Geobaldo
Analytical Chemistry
Valery N Konopsky, Elena V Alieva
Biosensors & Bioelectronics
Andrea M RossiThomas E Murphy
© 2021 Meta ULC. All rights reserved