Protease nexin I, a serpin, inhibits plasminogen-dependent degradation of muscle extracellular matrix

Muscle & Nerve
J S RaoB W Festoff

Abstract

Clonal, fusing, mouse skeletal muscle cells (C2) were grown to the myotube stage (90% confluence) before they were subjected to isotope-containing serum-free media (3H-proline or 35S-methionine). C2 myotubes secrete and organize a biosynthetically labeled matrix which adheres to the plastic after removal of myotubes with detergent and ammonium hydroxide. When these homotypic-labeled myotube matrices were incubated with myoblast-conditioned media containing high specific activity urokinase-type plasminogen activator, slow, but clearly detectable, release of label occurred. However, degradation of matrix, with solubilization of label, was accelerated sixfold by addition of human plasminogen to diluted myoblast-conditioned media. If protease nexin I, a cellular serine protease inhibitor purified from human fibroblast-conditioned media, was added (0.2 microgram/ml) with plasminogen, inhibition of matrix hydrolysis by 52% occurred. Higher concentrations (0.8 microgram/ml or above) of protease nexin 1 completely inhibited the degradation of extracellular matrix components. A similar protease inhibitor was purified from C2 myotube-conditioned media, and this molecule also inhibited the plasminogen-dependent release of extracellular ma...Continue Reading

References

Jun 1, 1978·The Journal of Experimental Medicine·E A Jaffe, D F Mosher
Jan 1, 1985·Advances in Cancer Research·K DanøL Skriver
Jan 1, 1987·Experimental Neurology·D Hantaï, B W Festoff
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B L BergmanJ B Baker
Jan 1, 1987·Progress in Brain Research·B W Festoff, D Hantaï
Nov 1, 1987·Journal of Cellular Physiology·R R MillerB W Festoff
Jun 1, 1985·The Journal of Clinical Investigation·A CampbellM Long
Jul 16, 1985·Biochemical and Biophysical Research Communications·J S RaoB W Festoff
Jun 1, 1974·Experimental Cell Research·J T Wachsman, J L Biedler
Jul 1, 1983·The Journal of Cell Biology·J A Madri, S K Williams
Nov 7, 1982·Journal of Theoretical Biology·M J BissellG Parry
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A Krystosek, N W Seeds
Feb 1, 1982·Journal of Cellular Physiology·B W FestoffK Romstedt
Jul 20, 1981·Brain Research·H Soreq, R Miskin
Sep 25, 1981·Science·A Krystosek, N W Seeds

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Citations

May 6, 1999·Journal of Cellular Physiology·C MbebiM Verdière-Sahuqué
Sep 19, 2000·Genes, Chromosomes & Cancer·W S ChangF Y Wu
Apr 1, 1994·Journal of Cellular Physiology·B W FestoffJ Chao
Sep 17, 1993·Brain Research. Developmental Brain Research·S L BirocJ M Fisher
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R R MillerB W Festoff
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D HantaïB W Festoff
Jun 27, 2009·BMC Cancer·Joanna Selzer-PlonLotte K Vogel
Sep 1, 1991·Progress in Cardiovascular Diseases·J HenkinH C Yang
Jan 1, 1994·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G J HoB W Festoff
Nov 1, 1994·The British Journal of Surgery·W G JiangM B Hallett
Dec 31, 2003·The Journal of Biological Chemistry·Patrick RossignolEduardo Anglés-Cano
Feb 16, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Maria DiamandisCatherine P M Hayward
Mar 30, 2019·Annals of the Rheumatic Diseases·Shuying ShenShunwu Fan
Apr 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Lian LiuJonathan M Peake
Oct 20, 2018·Current Medicinal Chemistry·Peng Xu, Mingdong Huang
Oct 2, 2021·Experimental and Therapeutic Medicine·Jicheng WangYangyang Zhang

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