Proteases from Vipera lebetina venom affecting coagulation and fibrinolysis

Haemostasis
J SiigurE Siigur

Abstract

Our studies of the venom from the Levantine viper Vipera lebetina have demonstrated the existence of both coagulants and anticoagulants in the same venom. We showed that V. lebetina venom contains: (1) proteases that degrade fibrinogen, but not fibrin; (2) fibrinolytic enzyme (lebetase); (3) factor X activator (VLFXA); (4) factor V activator (VLFVA). Fibrinolytic enzyme and VLFXA are metalloproteases; the other studied enzymes are serine proteases. alpha-Fibrinogenase has no homolog among known serine proteases. Beta-fibrinogenase is a typical thermostable arginine esterase that hydrolyzes esters and amides of arginine and attacks the beta-chain of fibrinogen. Lebetase is a direct-acting fibrinolytic zinc metalloendopeptidase related in amino acid sequence to reprolysins. We used the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique for the recovery and identification of peptides released by protease hydrolysis and for the detection of human factor X cleavage products after VLFXA hydrolysis. VLFXA cleaves the Arg(52)-Ile(53 )bond in the heavy chain of human factor X and the Arg(226)-Val(227) bond in human factor IX precursor; VLFVA cleaves Arg(1545)-Ser(1546) in factor V.

Citations

Sep 10, 2008·Hand : Official Journal of the American Association for Hand Surgery·Mehmet BozkurtEmin Kapi
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·R RajeshB S Vishwanath
Jun 19, 2012·The Journal of Biological Chemistry·Mettine H A Bos, Rodney M Camire
Jul 7, 2011·Current Opinion in Hematology·Rodney M Camire
Oct 5, 2010·Proteomics·Roberto GuerrantiRoberto Leoncini
Sep 29, 2004·Biochimica Et Biophysica Acta·Ene SiigurJüri Siigur
Mar 27, 2012·Toxicon : Official Journal of the International Society on Toxinology·Tihana KurtovićBeata Halassy
Sep 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Tihana KurtovićBeata Halassy
Mar 6, 2018·The Journal of Parasitology·Michael TesslerSebastian Kvist
Sep 10, 2013·The Journal of Biological Chemistry·Matthew W BunceRodney M Camire
Apr 30, 2003·The Journal of Biological Chemistry·Johannes A EbleUlrike Mayer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.