Proteases in Plasma and Kidney of db/db Mice as Markers of Diabetes-Induced Nephropathy.

ISRN Endocrinology
E Hadler-OlsenSvein Olav Kolset

Abstract

Db/db mice are overweight, dyslipidemic and develop diabetic complications, relevant for similar complications in human type 2 diabetes. We have used db/db and db/+ control mice to investigate alterations in proteinase expression and activity in circulation and kidneys by SDS-PAGE zymography, electron microscopy, immunohistochemistry, Western blotting, and in situ zymography. Plasma from db/db mice contained larger amounts of serine proteinases compared to db/+ mice. Kidneys from the db/db mice had a significantly larger glomerular surface area and somewhat thicker glomerular basement membranes compared to the db/+ mice. Furthermore, kidney extracts from db/+ mice contained metalloproteinases with M(r) of approximately 92000, compatible with MMP-9, not observed in db/db mice. These results indicate that higher levels of serine proteinases in plasma may serve as potential markers for kidney changes in db/db mice, whereas a decrease in MMP-9 in the kidney may be related to the glomerular changes.

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Citations

May 29, 2016·Biointerphases·Brooke L FarrugiaMegan S Lord
Jun 7, 2014·Journal of Cellular Physiology·Elin Hadler-OlsenLars Uhlin-Hansen
Mar 14, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ann Iren SolliElin Hadler-Olsen
Oct 30, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S O KolsetT Jenssen
Jun 13, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Trine M ReineSvein O Kolset
May 7, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Xiaohui TongGuanzhong Wu

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Methods Mentioned

BETA
zymography
biopsies

Software Mentioned

AnalySIS
Cell F

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