Proteasomal insensitivity of apoptin in tumor cells

Biochemical and Biophysical Research Communications
H LanzClaude Backendorf

Abstract

The small viral protein apoptin is capable of inducing apoptosis selectively in human tumor cells. In normal cells apoptin localizes in the cytoplasm where it forms aggregates, becomes epitope-shielded and eventually degraded. By inhibiting the proteasome activity with the chemical inhibitors bortezomib and Ada-Ahx(3)L(3)VS apoptin levels can be stabilized in normal cells similar to the tumor suppressor p53 protein. In contrast, proteasome inhibition in tumor cells did not affect the apoptin stability while it still stabilized p53 levels. Apparently, apoptin is degraded by proteasomal activity in normal human cells, a process that no longer takes place in tumor cells. This loss of proteasomal susceptibility appears to be specific for apoptin.

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Citations

Feb 9, 2013·Cell Death & Disease·H L LanzC Backendorf
Oct 26, 2012·Omics : a Journal of Integrative Biology·Paola PomaMonica Notarbartolo
Dec 12, 2018·BMC Cancer·Marina TerasSirje Rüütel Boudinot
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Waseem Akram MallaAshok Kumar Tiwari

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