Abstract
The peptidase activities of eukaryotic proteasomes are markedly activated by the 11 S REG or PA28. The three identified REG subunits, designated alpha, beta, and gamma, differ significantly in sequence over a short span of 15-30 amino acids that we call homolog-specific inserts. These inserts were deleted from each REG to produce the mutant proteins REGalphaDeltai, REGbetaDeltai, and REGgammaDeltai. The purified recombinant proteins were then tested for their ability to oligomerize and activate the proteasome. Both REGalphaDeltai and REGgammaDeltai formed apparent heptamers and activated human red cell proteasomes to the same extent as their full-length counterparts. By contrast, REGbetaDeltai exhibited, at low protein concentrations, reduced proteasome activation when compared with the wild-type REGbeta protein. REGbetaDeltai was able to form hetero-oligomers with a single site, monomeric REGalpha mutant and with REGalphaDeltai. At low concentrations, the REGalphaDeltai/REGbetaDeltai hetero-oligomers stimulated the proteasome less than REGalpha/REGbeta oligomers formed from wild-type subunits, and the reduced activation by REGalphaDeltai/REGbetaDeltai was due to removal of the REGbeta insert, not the REGalpha insert. These stu...Continue Reading
References
Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Sep 23, 1991·FEBS Letters·A GrziwaF Kopp
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Dec 4, 1995·FEBS Letters·V UstrellM Rechsteiner
Mar 1, 1995·Immunity·G NiedermannK Eichmann
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
Jan 1, 1993·Enzyme & Protein·A LupasW Baumeister
Apr 28, 1995·Science·J LöweR Huber
Apr 28, 1995·Science·E SeemüllerW Baumeister
Jul 11, 1994·FEBS Letters·C RealiniM Rechsteiner
Jan 1, 1996·Current Topics in Cellular Regulation·L Hoffman, M Rechsteiner
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Sep 1, 1996·Immunology Today·M GroettrupP M Kloetzel
Apr 3, 1997·Nature·M GrollR Huber
Jan 1, 1997·Advances in Immunology·K TanakaN Shimbara
Mar 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N TanahashiK Tanaka
Nov 5, 1997·The Journal of Biological Chemistry·C RealiniM Rechsteiner
Nov 5, 1997·The Journal of Biological Chemistry·X SongG N DeMartino
Dec 24, 1997·Nature·J R KnowltonC P Hill
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z ZhangM Rechsteiner
Citations
Apr 11, 2001·Biochimie·J Li, M Rechsteiner
Feb 17, 2000·Gene·R W WestE Milgrom
Dec 22, 1999·The Biochemical Journal·M RechsteinerV Ustrell
Jul 4, 2001·The EMBO Journal·J LiM Rechsteiner
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Apr 4, 2003·Biological Chemistry·Ralf StohwasserPeter M Kloetzel
Aug 12, 2006·Molecular Immunology·Guozhu LiuXinhua Chen
Mar 19, 2014·Chemistry & Biology·Mary RaulePaolo Cascio
Feb 5, 2013·Biochemical and Biophysical Research Communications·Masaaki SugiyamaKoichi Kato
Jun 3, 2000·Journal of Molecular Biology·J LiM Rechsteiner
Apr 28, 2004·Archives of Biochemistry and Biophysics·Xiaolin GaoMartin Rechsteiner
Nov 7, 1998·The Journal of Biological Chemistry·Z ZhangM Rechsteiner
Mar 27, 2019·Development·Hyojeong HwangJing Yang
Oct 12, 2000·The Journal of Biological Chemistry·P MassonP Young
Nov 24, 1999·The Journal of Biological Chemistry·Y YaoC C Wang
Aug 7, 2019·Nature Microbiology·Stanley C XieLeann Tilley
Feb 11, 2021·Biomolecules·Paolo Cascio