Proteasome activator REGγ promotes inflammation in Leydig cells via IkBε signaling

International Journal of Molecular Medicine
Tiancheng XieYunfei Xu

Abstract

The development of testicular inflammation affects the normal male reproductive function. The proteasome activator complex subunit 3 (REGγ) has been suggested to regulate experimental colitis. However, to the best of our knowledge, a potential association between REGγ and testicular inflammation has not been demonstrated. The present study successfully established inflammatory models in C57 mice, primary Leydig cells and the TM3 cell line. It was observed that the absence of REGγ conveyed a significantly protective effect toward testosterone secretion in Leydig cells. REGγ deficiency significantly decreased the expression levels of phosphorylated transcription factor p65 and inflammatory factors in testis tissues, primary Leydig cells and the TM3 cell line. Inflammation also upregulated the expression levels of REGγ. Furthermore, the degradation of the nuclear factor light‑chain‑enhancer of activated B cells (NF‑κB) inhibitor ε (IkBε) signaling pathway regulated REGγ and NF‑κB expression. Double knockdown of REGγ and IkBε restored the response in wild‑type cells to LPS‑induced inflammation. In summary, these results demonstrated that REGγ regulates NF‑κB activity by specifically degrading IkBε to regulate inflammation in testic...Continue Reading

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Citations

Jun 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Chen GaoJia-Ming Sun

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Methods Mentioned

BETA
fluorescence microscopy
protein collection
PCR
ELISA

Software Mentioned

FlowJo
Pro Plus
Image
SPSS

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