PMID: 7525280Oct 15, 1994Paper

Proteasome-associated RNAs are non-specific

European Journal of Biochemistry
V PamnaniW Baumeister

Abstract

The RNA isolated from RNase-treated proteasome preparations from human erythrocytes, HeLa cells, the archaeon Thermoplasma acidophilum and also from recombinant proteasomes of T. acidophilum expressed in Escherichia coli was characterized. The RNA associated with structurally similar protein particles, namely with the two molecular chaperones, groEL from E. coli and with the thermosome from T. acidophilum, served as controls. Electrophoretic analysis on polyacrylamide gels of the radioactively end-labelled RNA revealed a very similar size distribution pattern, irrespectively of the protein particles from which they had been isolated. The predominant RNA species were in the size ranges 80 nucleotides and 120 nucleotides, respectively. Partial sequencing of their terminal regions by mobility-shift analysis revealed that, of the proteasomes from human erythrocytes, the approximately 80-nucleotide-long RNA consists of a heterogenous population of mostly tRNA species because they carried the tRNA-specific 3'-terminal sequence motif 5'-CCA-3'. The RNA in the size range 120 nucleotides isolated from the proteasomes of human erythrocytes and of T. acidophilum was also heterogeneous and displayed, in the terminal regions, a remarkable s...Continue Reading

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Citations

Jan 1, 1995·Molecular Biology Reports·H P SchmidY Briand
May 11, 2002·Journal of Cellular and Molecular Medicine·Cezary Wójcik
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M BochtlerR Huber
Sep 7, 2014·Biochimica Et Biophysica Acta·David S PitcherMaurits F Kleijnen
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Rongbing HuangXing Chen
Nov 30, 2000·Archives of Biochemistry and Biophysics·M Orlowski, S Wilk
Nov 5, 1999·Journal of Molecular Biology·I GutscheW Baumeister

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