Proteasome-dependent degradation of alpha-catenin is regulated by interaction with ARMc8alpha

The Biochemical Journal
Takeyuki SuzukiYoshiaki Ishigatsubo

Abstract

ARMc8 (armadillo-repeat-containing protein 8) is a key component of the CTLH (C-terminal to lissencephaly type-1-like homology motif) complex in mammalian cells. This complex is well conserved in Saccharomyces cerevisiae and has been characterized as a FBPase (fructose-1, 6-bisphosphatase)-degrading complex. The yeast homologue of ARMc8, Gid (glucose-induced degradation) 5p, plays an essential role in the ubiquitin- and proteasome-dependent degradation of FBPase. To elucidate the function of ARMc8, we used a yeast two-hybrid system to screen a human skeletal muscle cDNA library. alpha-Catenin was isolated as a binding protein of ARMc8alpha. This association was confirmed by co-immunoprecipitation assay using MDCK (Madin-Darby canine kidney) cells in which exogenous alpha-catenin and ARMc8alpha were overexpressed. The association was also confirmed by co-immunoprecipitation assay using endogenous proteins in untransfected MDCK cells. We then used immunofluorescence microscopy of MDCK cells and C2C12 cells to investigate the intracellular distribution of ARMc8. Exogenously expressed ARMc8 was co-localized with alpha-catenin and beta-catenin along the cell membrane, suggesting an association between alpha-catenin and ARMc8 in the ...Continue Reading

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Citations

Aug 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chuifeng FanEnhua Wang
Mar 6, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yang ZhaoChaoliu Dai
Jun 18, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guiyang JiangEnhua Wang
Nov 14, 2012·Neurological Research·D M F MendonçaA M B Martinez
Jun 5, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chengyao XieEnhua Wang
Aug 9, 2016·Cellular and Molecular Life Sciences : CMLS·Ismail Sahin GulFrans van Roy
Jul 12, 2018·PLoS Genetics·Lucia Carolina Leal-EstebanDaniel B Constam
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Apr 22, 2016·FEBS Letters·Toshiyasu GotoHiroshi Shibuya
Dec 31, 2019·Journal of Oncology·Nickelas HuffmanVincenzo Coppola
Jul 1, 2017·Open Biology·Louisa M SalemiCaroline Schild-Poulter
Mar 31, 2015·Developmental Biology·Jennifer E BestmanHollis T Cline

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