Proteasome mediated degradation of CDC25C and Cyclin B1 in Demethoxycurcumin treated human glioma U87 MG cells to trigger G2/M cell cycle arrest

Toxicology and Applied Pharmacology
Neetika LalPratibha Mehta Luthra

Abstract

Recently, we have reported that Demethoxycurcumin induced Reactive oxygen species via inhibition of Mitochondrial Superoxide Dismutase is an initial event to trigger apoptosis through caspase-8 and 9 activation and to inhibit Akt/NF-κB survival signaling in human glioma U87 MG cells (Kumar et al., 2018). Although cell-cycle disruption had been suggested to be the possible mechanism for DMC inhibitory effect on human glioma U87 MG cells, comprehensive mechanisms of cell-cycle arrest caused by DMC are not fully understood. The present study was designed to elucidate the DMC induced mechanism of cell cycle arrest in human glioma U87 MG cells. In this study, the results illustrated that DMC induced Reactive oxygen species (ROS) leads to reduced expression of CDC25C, Cyclin B1 and CDK1 (Thr161) triggers G2/M cell cycle arrest in U87 MG glioma cells. Moreover, the DMC induced ROS generation activates ubiquitination and proteasome degradation of CDC25C and Cyclin B1 in U87MG glioma cells. In addition, the immunoprecipitation results showed that significant dissociation of CDK1or CDC2-Cyclin B1 complex leads to G2/M cell cycle arrest. To explore the possibility of direct involvement of DMC in the dissociation of CDK1/Cyclin B1 complex,...Continue Reading

Citations

Aug 1, 2020·Organic & Biomolecular Chemistry·Ranjay ShawRamendra Pratap
Jun 7, 2019·Cancer Control : Journal of the Moffitt Cancer Center·Qi WangYong Chen
Mar 2, 2019·Cancer Cell International·Wen-Xiu DingXin-Chen Sun
Oct 23, 2019·Signal Transduction and Targeted Therapy·Mrinal K GhoshMalini Basu
May 30, 2020·The Journal of International Medical Research·Er Chen, Renzhi Pei
Dec 18, 2019·Journal of Cancer Research and Clinical Oncology·Moiken MehnerJanka Held-Feindt
Jan 14, 2020·European Journal of Medicinal Chemistry·Zahra ShabaninejadHamed Mirzaei
Oct 12, 2020·Signal Transduction and Targeted Therapy·Mrinal K GhoshMalini Basu
Sep 2, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·Z AlmalkiM A Zamzami

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