Protection against acetaminophen-induced acute liver failure by omentum adipose tissue derived stem cells through the mediation of Nrf2 and cytochrome P450 expression

Journal of Biomedical Science
Yu-Jen HuangYao-Ming Wu

Abstract

Acetaminophen (APAP) overdose causes acute liver failure (ALF) in animals and humans via the rapid depletion of intracellular glutathione (GSH) and the generation of excess reactive oxygen species (ROS) that damage hepatocytes. Stem cell therapy is a potential treatment strategy for ALF. We isolated mesenchymal stem cells (MSCs) from mice omentum adipose tissue-derived stem cells (ASCs) and transplanted them into a mouse model of APAP-induced ALF to explore their therapeutic potential. In addition, we performed in vitro co-culture studies with omentum-derived ASCs and primary isolated hepatocytes to demonstrate the hepatoprotective effect of omentum-derived ASCs on hepatocytes that were subjected to APAP-induced damage. ASC transplantation significantly improved the survival rate of mice with ALF and attenuated the severity of APAP-induced liver damage by suppressing cytochrome P450 activity to reduce the accumulation of toxic nitrotyrosine and the upregulation of NF-E2-related factor 2 (Nrf2) expression, resulting in an increase in the subsequent antioxidant activity. These effects protected the hepatocytes from APAP-induced damage through the suppression of downstream MAPK signal activation and inflammatory cytokine productio...Continue Reading

References

May 17, 1996·The Journal of Biological Chemistry·S S LeeF J Gonzalez
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·K ChanY W Kan
May 30, 2001·Trends in Molecular Medicine·A I Caplan, S P Bruder
Nov 20, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laura P JamesJack A Hinson
Nov 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kuan-Der LeeOscar Kuang-Sheng Lee
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hyon-Jeen Kim, Andre E Nel
May 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhang-Xu LiuNeil Kaplowitz
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yao-Ming WuSanjeev Gupta
Aug 29, 2007·Clinics in Liver Disease·Anne M Larson
Jul 19, 2008·The New England Journal of Medicine·Kennon J Heard
Apr 11, 2009·Critical Care : the Official Journal of the Critical Care Forum·Runkuan YangMitchell P Fink
Aug 8, 2009·Biochemical Pharmacology·Mohamed A AbdelmegeedByoung-Joon Song
Jun 8, 2010·Cell Transplantation·Hiroshi YagiMartin L Yarmush
Sep 8, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emira AyroldiCarlo Riccardi
Oct 6, 2012·Cell Biology International·Kyung-Ah ChoKyung-Ha Ryu
Mar 7, 2013·Pharmaceutical Research·Mitchell R McGill, Hartmut Jaeschke
Jan 5, 2014·Toxicological Research·Sang Il Gum, Min Kyung Cho
Aug 16, 2014·BioResearch Open Access·Sébastien SartYan Li

❮ Previous
Next ❯

Citations

Feb 20, 2019·Cellular and Molecular Neurobiology·Xiao-Qing LiGuang-Zhi Liu
Feb 18, 2020·Toxicology Mechanisms and Methods·Zixiong ZhouBumseok Kim
Jul 10, 2019·Stem Cell Research & Therapy·Chenxia HuLanjuan Li
Jan 24, 2019·Frontiers in Pharmacology·Dongwei XuXiaoni Kong
Nov 7, 2019·Journal of Cellular and Molecular Medicine·Chenxia HuLanjuan Li
Aug 26, 2018·Stem Cell Research & Therapy·Yong-Hong WangHong Tang
Jun 15, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Chenxia Hu, Lanjuan Li
Aug 28, 2021·Journal of Reproductive Immunology·Narges NouriMehdi Yousefi

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
protein assay
ELISA

Software Mentioned

ImageJ
SigmaStat
GraphPad InStat

Related Concepts

Related Feeds