Protection against anoikis and down-regulation of cadherin expression by a regulatable beta-catenin protein

The Journal of Biological Chemistry
Zhigang WengJackie Papkoff

Abstract

beta-Catenin signaling plays a key role in a variety of cellular contexts during embryonic development and tissue differentiation. Aberrant beta-catenin signaling has also been implicated in promoting human colorectal carcinomas as well as a variety of other cancers. To study the molecular and cellular biological functions of beta-catenin in a controlled fashion, we created a regulatable form of activated beta-catenin by fusion to a modified estrogen receptor (ER) ligand binding domain (G525R). Transfection of tissue culture cells with expression vectors encoding this hybrid protein allows the signal transduction function of beta-catenin to be induced by the synthetic estrogen, 4-hydroxytamoxifen, leading to regulated activation of a beta-catenin-lymphocyte enhancer-binding factor-dependent reporter gene as well as induction of endogenous cyclin D1 expression. The activation of ER-beta-catenin signaling rescues RK3E cells from anoikis and correlates with an increased phosphorylation of mitogen-activated protein kinase. The inhibition of anoikis by ER-beta-catenin can be abolished by a mitogen-activated protein kinase pathway inhibitor, PD98059. Evidence is also provided to show that ER-beta-catenin down-regulates cadherin prote...Continue Reading

References

Sep 17, 1992·Nature·S M PowellK W Kinzler
Nov 1, 1988·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M TakeichiA Nagafuchi
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·D L RimmJ S Morrow
Jan 1, 1994·Methods in Cell Biology·T MattioniD Picard
Sep 30, 1994·Science·H Hermeking, D Eick
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
May 20, 1994·Cell·E Ruoslahti, J C Reed
Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Dec 10, 1993·Science·B RubinfeldP Polakis
Apr 1, 1993·Trends in Biochemical Sciences·E Nishida, Y Gotoh
May 1, 1996·Molecular and Cellular Biology·J PapkoffP Polakis
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·P J MorinK W Kinzler
Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Oct 1, 1996·Trends in Genetics : TIG·P W Ingham
Oct 1, 1996·Current Opinion in Cell Biology·O HuberR Kemler

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Citations

Dec 23, 2003·Immunology Letters·Marek JakóbisiakJakub Gołab
Jan 22, 2003·Biochemical and Biophysical Research Communications·Gerard BainJackie Papkoff
Mar 1, 2014·Neurotoxicity Research·Hong LiuRichard Salvi
Apr 13, 2004·Scandinavian Journal of Gastroenterology·J B Seidelin
Apr 25, 2012·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Shiuan-Shinn LeeYu-Chao Chang
May 29, 2012·Biochemical and Biophysical Research Communications·Lei JiangKwok-Pui Fung
Nov 21, 2015·BioMed Research International·Sharan Malagobadan, Noor Hasima Nagoor
Jul 1, 2005·Journal of Cellular Biochemistry·James K Wahl
Nov 7, 2002·Oncogene·T Christopher WindhamGary E Gallick
Jul 7, 2017·International Journal of Colorectal Disease·Jutta Maria NagelFrank Thomas Kolligs
Oct 13, 2006·American Journal of Physiology. Cell Physiology·Fernando NeriaMaría Angeles Castilla
Feb 16, 2006·Journal of Cell Science·Ki-Sook ParkKang-Yell Choi
Oct 10, 2002·Experimental Cell Research·Thomas MüllerJackie Papkoff

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