Protection Against Chlamydia trachomatis Infection and Upper Genital Tract Pathological Changes by Vaccine-Promoted Neutralizing Antibodies Directed to the VD4 of the Major Outer Membrane Protein

The Journal of Infectious Diseases
Anja W OlsenPeter Andersen

Abstract

The VD4 region from the Chlamydia trachomatis major outer membrane protein contains important neutralizing B-cell epitopes of relevance for antibody-mediated protection against genital tract infection. We developed a multivalent vaccine construct based on VD4s and their surrounding constant segments from serovars D, E, and F. Adjuvanted with cationic liposomes, this construct promoted strong immune responses to serovar-specific epitopes, the conserved LNPTIAG epitope and neutralized serovars D, E, and F. Vaccinated mice were protected against challenge, with protection defined as reduced bacterial numbers in vagina and prevention of pathological changes in the upper genital tract. Adoptive transfer of serum and T-cell depletion experiments demonstrated a dominant role for antibodies and CD4(+) T cells in the protective immune response. Integrating a multivalent VD4 construct into the sequence of the major outer membrane protein resulted in a protective and broadly neutralizing vaccine. Our findings emphasize the important role of antibodies in protection against Chlamydia trachomatis.

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Mar 5, 2016·Expert Review of Vaccines·Hong YuRobert C Brunham
Nov 12, 2016·Human Vaccines & Immunotherapeutics·Steven LiangJames Mahony
Mar 8, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marco GrasseJes Dietrich
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Sep 8, 2016·Infection and Immunity·Elizabeth K NaglakRichard P Morrison
Jan 10, 2019·ILAR Journal·Signe Tandrup SchmidtDennis Christensen
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Oct 20, 2020·Frontiers in Immunology·Gabriel Kristian PedersenDennis Christensen
Dec 5, 2020·Vaccines·Marijana StojanovicAleksandra Inic-Kanada
Mar 9, 2021·Expert Review of Vaccines·Luis M de la MazaSukumar Pal
Mar 28, 2021·Vaccine·Sam M Murray, Paul F McKay

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