Protection against collagen-induced arthritis by electrotransfer of an expression plasmid for the interleukin-4

Biochemical and Biophysical Research Communications
Seong-Hyun HoJong-Mook Kim

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, leading to cartilage and bone destruction. We investigated whether the electrotransfer of IL-4 DNA could regulate the disease progress of murine collagen-induced arthritis (CIA). The maximum serum level of mIL-4 was measured by 340 pg/ml on day 1 following DNA transfer. The onset of severe CIA and the degree of synovitis and cartilage erosion were significantly reduced in mice treated with IL-4 DNA (P<0.05). The beneficial effect of IL-4 gene transfer lasted for at least 17 days subsequent to treatment. The expression of IL-1beta was considerably decreased in the paws by IL-4 DNA transfer (P<0.01). On the contrary, the ratio of TIMP2 to MMP2 significantly increased in the IL-4 DNA-treated group (P<0.01). These data demonstrated that electroporation-mediated gene transfer could provide a new approach as an IL-4 therapy for autoimmune arthritis.

References

Dec 1, 1992·Current Opinion in Immunology·F M Brennan, M Feldmann
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G J ThorbeckeM A Palladino
Mar 1, 1992·Clinical Rheumatology·P M Kaklamanis
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P H HartJ A Hamilton
Jun 26, 1998·Annals of the Rheumatic Diseases·C J JacksonM Nguyen
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·G RizzutoE Fattori
May 26, 2001·The Journal of Clinical Investigation·Y MoritaD A Fox
Jun 2, 2001·Human Gene Therapy·M Nishikawa, L Huang

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Citations

Jan 8, 2008·Modern Rheumatology·Russell S Traister, Raphael Hirsch
Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
Aug 24, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Miho SukedaiTatsuji Nishihara
Nov 6, 2010·Biomedical Engineering Online·Anze ZupanicMojca Pavlin
Nov 16, 2007·Arthritis Research & Therapy·Miguel Otero, Mary B Goldring
Mar 4, 2008·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Makoto NakasatoHitoshi Kohsaka
Mar 28, 2006·Biochemical and Biophysical Research Communications·Seong-Hyun HoJong-Mook Kim
May 11, 2005·Biochemical and Biophysical Research Communications·Kyoung Chul ParkMirim Jin
Jun 5, 2007·Journal of Cellular Biochemistry·Mohammed El MabroukMuhammad Zafarullah

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