PMID: 7514676Jun 1, 1994Paper

Protection against lethal lymphocytic choriomeningitis virus (LCMV) infection by immunization of mice with an influenza virus containing an LCMV epitope recognized by cytotoxic T lymphocytes

Journal of Virology
M R CastrucciY Kawaoka

Abstract

The reverse genetics system has made it possible to modify the influenza virus genome. By this method, we were able to assess influenza virus as a vaccine vector for protecting BALB/c mice against otherwise lethal lymphocytic choriomeningitis virus (LCMV) infection. A single dose of influenza virus [A/WSN/33 (H1N1)] bearing a cytotoxic T-lymphocyte-specific epitope of the LCMV nucleoprotein (residues 116 to 127) in the neuraminidase stalk protected mice against LCMV challenge for at least 4 months. The immunity was mediated by cytotoxic T lymphocytes and was haplotype specific, indicating that the observed protective response was solely a consequence of prior priming with the H-2d LCMV nucleoprotein epitope expressed in the recombinant influenza virus. We also found that as many as 58 amino acids could be inserted into the neuraminidase stalk without loss of viral function. These findings demonstrate the potential of influenza virus as a vaccine vector, with the neuraminidase stalk as a repository for foreign epitopes.

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M B OldstoneJ L Whitton
Jan 1, 1992·Annual Review of Immunology·P C DohertyS R Carding
Nov 1, 1991·Virology·M EnamiP Palese
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·W M KastC J Melief
May 1, 1991·Journal of Virology·M Enami, P Palese
Jan 1, 1990·The Journal of Infectious Diseases·L PrevecF L Graham
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M EnamiP Palese
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M G MurrayE Wimmer
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J E MorinP P Hung
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Sep 1, 1984·The Journal of Experimental Medicine·A E LukacherT J Braciale
Sep 1, 1983·European Journal of Immunology·P M Taylor, B A Askonas
Aug 1, 1983·The Journal of General Virology·F J Dutko, M B Oldstone
Feb 1, 1993·Journal of Virology·M R Castrucci, Y Kawaoka

❮ Previous
Next ❯

Citations

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·P Palese
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yutaka FujiiYoshihiro Kawaoka
Aug 26, 2016·Current Opinion in HIV and AIDS·Hendrik Streeck
Feb 10, 1997·AIDS Research and Human Retroviruses·G L Ada, M J McElrath
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·C SedlikC Leclerc
May 14, 2004·Journal of Virology·Masato HattaYoshihiro Kawaoka
Aug 28, 2012·Bioengineered·Junwei LiMingtao Zeng
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·P G StevensonP C Doherty
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·G T BelzP C Doherty
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Apr 19, 2013·Clinical and Experimental Vaccine Research·Jae-Min SongBaik-Lin Seong
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda M WakimFrancis R Carbone
Feb 20, 2019·Expert Review of Vaccines·Thomas GerlachGuus F Rimmelzwaan
May 1, 1995·Journal of Virology·R AltmeyerM F Saron

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.