Protection against Mucosal SHIV Challenge by Peptide and Helper-Dependent Adenovirus Vaccines.

Viruses
Eric A WeaverMichael A Barry

Abstract

Groups of rhesus macaques that had previously been immunized with HIV-1 envelope (env) peptides and first generation adenovirus serotype 5 (FG-Ad5) vaccines expressing the same peptides were immunized intramuscularly three times with helper-dependent adenovirus (HD-Ad) vaccines expressing only the HIV-1 envelope from JRFL. No gag, pol, or other SHIV genes were used for vaccination. One group of the FG-Ad5-immune animals was immunized three times with HD-Ad5 expressing env. One group was immunized by serotype-switching with HD-Ad6, HD-Ad1, and HD-Ad2 expressing env. Previous work demonstrated that serum antibody levels against env were significantly higher in the serotype-switched group than in the HD-Ad5 group. In this study, neutralizing antibody and T cell responses were compared between the groups before and after rectal challenge with CCR5-tropic SHIV-SF162P3. When serum samples were assayed for neutralizing antibodies, only weak activity was observed. T cell responses against env epitopes were higher in the serotype-switched group. When these animals were challenged rectally with SHIV-SF162P3, both the Ad5 and serotype-switch groups significantly reduced peak viral loads 2 to 10-fold 2 weeks after infection. Peak viral loa...Continue Reading

References

Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·K MitaniS Kochanek
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B WangD B Weiner
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J ParksF L Graham
Mar 1, 1997·Journal of Virology·S HardyM L Phipps
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·N MorralA L Beaudet
Jun 6, 2000·Advanced Drug Delivery Reviews·J W Simecka
Apr 18, 2003·AIDS·Thomas Lehner, Peter A Anton
Nov 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Donna Palmer, Philip Ng
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arguinaldo R PintoHildegund C J Ertl
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dan H BarouchJaap Goudsmit

❮ Previous
Next ❯

Citations

Jan 22, 2011·Virology·Eric A WeaverMichael A Barry
Nov 28, 2013·Human Vaccines & Immunotherapeutics·Eric A Weaver
Dec 5, 2014·Journal of Virology·Hui-Wen ChangDan H Barouch
Dec 19, 2017·Expert Review of Vaccines·Michael Barry

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
transfection

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.